The following anti-tau antibodies were used: HT7 (human-specific Tau; Invitrogen, #MN-1000), Tau-5 (total tau; Biosource, #AHB0042), Tau-12 (total tau; generously provided by Dr. Lester Binder, Northwestern University, IL), TG5 and DA9 (total tau; generously provided by Dr. Peter Davies, Albert Einstein College of Medicine, NY), p-Tau Ser396 (Invitrogen, #44-752G), PHF-1 (p-Ser396/404; Davies), CP13 (p-S202; Davies), AT100 (p-Ser212/214; Pierce, #P10636), AT180 (pThr231/Ser235; Innogenetics, #90337), 12E8 (p-Ser262/356; generously provided by Dr. Peter Seubert, Elan Pharmaceuticals), Tau-1 (dephosphorylated Ser195/198/199/202; Chemicon, #MAB3420), MC-1 (conformation dependent antibody; Davies), LC3 (Novus Biologicals, #NB600–1384), SQSTM1 (Abnova, #H00008878-M01), pALK (p-Tyr1604; Cell signaling, #3341), ALK1 (BD Pharmingen, #559254), ALK (Invitrogen, #51–3900), ALK (DAKO, #IS64130–2), α-tubulin (Sigma, #T5168), β-actin (Sigma, #A2668), and GFP (Santa Cruz, #sc-8334). Anti-ALK monoclonal antibodies, mAb46, mAb30, and mAb13, generously provided from Dr. Marc Vigny (INSERM U440, Paris) [31 (link)].
At180
Manufactured by Fujirebio
Sourced in Belgium
The AT180 is a laboratory equipment product designed for research and analytical applications. It is a precise and reliable instrument that performs specific functions. The core function of the AT180 is to [DESCRIPTION NOT AVAILABLE].
Automatically generated - may contain errors
Lab products found in correlation
α tubulin, by Merck Group (1 mentions)
Ptau ser396, by Thermo Fisher Scientific (1 mentions)
β actin, by Merck Group (1 mentions)
P0448, by Agilent Technologies (1 mentions)
Bc100 494, by Novus Biologicals (1 mentions)
P0161, by Agilent Technologies (1 mentions)
At100, by Fujirebio (1 mentions)
Tomm20, by Santa Cruz Biotechnology (1 mentions)
Lamp1, by BD (1 mentions)
2 protocols using at180
1
Antibody Panel for Tau Pathology
2
Tau Protein Immunodetection in Neurodegeneration
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The primary antibodies used were: Tau5 (577801, Calbiochem), Tau404 (44758G, Life Technologies), Tau396 (44752G, Life Technologies), PHF1 [17] (a kind gift of Dr. P. Davies, Albert Einstein Coll., Bronx, NY, USA), Tau7.51 [18] (a kind gift of Dr. C. Wischik, MRC, Cambridge, UK), AT8 (MN1020, Innogenetics, Ghent, Belgium), AT100 (MN1060, Innogenetics), AT180 (MN1040, Innogenetics), -Actin (A2228, Sigma-Aldrich), APP (A8717, Sigma-Aldrich), -Amyloid (803015, Biolegend), TOMM20 (sc-11415, Santa Cruz), LC3 (B7931, Sigma-Aldrich), p62 (610832, BD Bioscience), LAMP1 (611042, BD Bioscience), CatB (AB4064, Chemicon), GAPDH (ab8245, Abcam), PARK2 (sc-32282, Santa Cruz), PINK1 (BC100-494, Novus), COX4 (sc-69359, Santa Cruz). The secondary antibodies for western blot studies were horseradish peroxidase-conjugated anti-rabbit or anti-mouse IgGs (P0448 and P0161, DAKO) and for immunofluorescence were anti-mouse or anti-rabbit IgGs alexa-488 or -555 labeled (Molecular Probes, Millipore).
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