Lc3ii 14600 1 ap

Manufactured by Proteintech

LC3II (14600-1-AP) is a primary antibody that recognizes the microtubule-associated protein 1A/1B-light chain 3 (LC3). It is used to detect the lipidated form of LC3, known as LC3-II, which is a widely used marker for autophagy.

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Lab products found in correlation

Nfe2l2 16396 1 ap, by Proteintech (1 mentions) Mitotracker, by Beyotime (1 mentions) Lyso tracker red, by Solarbio (1 mentions) Crystal violet staining solution, by Solarbio (1 mentions) Glutathione (gsh), by NJJCBIO (1 mentions) Cy3 labeled goat anti rabbit igg h l a0516, by Beyotime (1 mentions) Cleaved il 1β 83186, by Cell Signaling Technology (1 mentions) Nlrp3 15101, by Cell Signaling Technology (1 mentions) Cy3 labeled goat anti mouse igg h l a0521, by Beyotime (1 mentions) Ab180799, by Abcam (1 mentions) Hrp conjugated affinipure goat anti rabbit igg sa00001 2, by Proteintech (1 mentions) Gapdh 60004 1 ig, by Proteintech (1 mentions) Ab93015, by Abcam (1 mentions)

2 protocols using lc3ii 14600 1 ap

Multimodal Assessment of Cellular Stress

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NFE2L2 (16396-1-AP) and LC3II (14600-1-AP) were purchased from proteintech. HO-1 (86806, CST), FTH1 (4393, CST), Gpx4 (PA5-102521, Invitrogen), NCOA4 (HC071-1F11, Sigma-Aldrich).
Esculetin, Cisplatin and Sorafenib (MCE, USA), T-AOC Assay, ROS Assay Kit, Crystal Violet Staining Solution, Lyso-Tracker Red (Solarbio, China), Mito-Tracker (Beyotime, China), DPPH free radical scavenging rate detection kit (Leagene, China), Transwell^TM polycarbonate membrane cell culture inserts (Corning, Germany), Liperfluo, FerroOrange, Cell Counting Kit-8 (DOJINDO, Japan), GSH (reduced glutathione) determination kit (NJJCBIO).

Xiu Z., Li Y., Fang J., Han J., Li S., Li Y., Yang X., Song G., Li Y., Jin N., Zhu Y., Zhu G., Sun L, & Li X. (2023). Inhibitory Effects of Esculetin on Liver Cancer Through Triggering NCOA4 Pathway-Mediation Ferritinophagy in vivo and in vitro. Journal of Hepatocellular Carcinoma, 10, 611-629.

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Antibody Sources for Cellular Assays

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Antibodies against CDKN1A (10355-1-AP), interleukin (IL)-18 (10663-1-AP), cleaved poly (ADP-ribose) polymerase (C-PARP, 13371-1-AP), glutathione peroxidase 4 (GPX4, 67763-Ig), HMGB1 (10829-1-AP), lipidation of microtubule-associated protein 1 light chain 3 form II (LC3-II, 14600-1-AP), P53BP1 (20002-1-AP), and GAPDH (60004-1-Ig) were obtained from Proteintech (Wuhan, China). Antibodies against caspase-3 (CASP-3, D3R6Y), cleaved-IL-1β (83186), γH2AX (9718), nucleotide-binding and oligomerization domain-like receptor (NLR) pyrin domain-containing 3 (NLRP3, 15101), and cleaved-caspase-1 (89332) were obtained from Cell Signaling Technology (Danvers, MA, USA). Antibodies against absent in melanoma 2 (AIM2, ab93015), gasdermin-D (GSDMD)-N (ab215203), and apoptosis-associated speck-like protein containing a C-terminal caspase recruitment domain (ASC, ab180799) were purchased from Abcam (Massachusetts, UK). Horseradish peroxidase (HRP)-conjugated AffiniPure goat anti-mouse IgG (SA00001-1) and HRP-conjugated AffiniPure goat anti-rabbit IgG (SA00001-2) were purchased from Proteintech. Cy3-labeled goat anti-mouse IgG (H + L) (A0521) and Cy3-labeled goat anti-rabbit IgG (H + L) (A0516) were purchased from Beyotime (Shanghai, China).

Li J., Liu T., Tang N., Lin S., Zhang F., Yuan W., Zhang T., Deng S.H., Wu D.M, & Xu Y. (2024). Cyclin-dependent kinase inhibitor 1A inhibits pyroptosis to enhance human lung adenocarcinoma cell radioresistance by promoting DNA repair. Heliyon, 10(5), e26975.

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