Mouse 610201

Manufactured by Cell Signaling Technology

The Mouse 610201 is a laboratory equipment product designed for experimental use. It serves as a tool for research purposes. The core function of this product is to facilitate specific experimental protocols, but a detailed description cannot be provided while maintaining an unbiased and factual approach.

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Lab products found in correlation

Rabbit anti tom20, by Santa Cruz Biotechnology (2 mentions) Rabbit anti mitofusin 2, by Merck Group (2 mentions) Ar a014418, by Abcam (2 mentions) Ct99021, by Abcam (2 mentions) Mouse anti α tubulin dm1a, by Merck Group (1 mentions) Rabbit anti gfp and mouse anti βactin, by Abcam (1 mentions) Rabbit anti tdp 43 12892 1 ap, by Proteintech (1 mentions) Mouse pdi rl77, by Thermo Fisher Scientific (1 mentions) Antibodies to total and ser9 phosphorylated inactive gsk3β, by BD (1 mentions) Rabbit anti glyceraldehyde 3 phosphate dehydrogenase gapdh, by Cell Signaling Technology (1 mentions) Mouse anti myc 9b11, by Cell Signaling Technology (1 mentions) Rabbit anti gfp ab290, by Abcam (1 mentions)

2 protocols using mouse 610201

Generation of Rat and Rabbit Antibodies to VAPB and PTPIP51

Cited 1 time

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Rat and rabbit antibodies to VAPB and PTPIP51 were generated by immunisation with GST-VAPB(1-220) and GST-PTPIP51(36-470) respectively^{20 (link)}. Rabbit anti-PTPIP51 was from Atlas, rabbit anti-hemagglutin (HA), mouse anti-α-tubulin (DM1A) and rabbit anti-Mitofusin2 were from Sigma. Rabbit anti-TOM20 was from Santa Cruz Biotechnology. Rabbit anti-GFP and mouse anti-βactin were from Abcam. Mouse PDI (RL77) was from Affinity Bioreagents. Rabbit anti-TDP-43 (12892-1-AP) was from Proteintech. Antibodies to total and ser9 phosphorylated (inactive) GSK3β were from BD Transduction Labs (mouse 610201) and Cell Signalling (rabbit 9336) respectively. The concentrations used for the different antibodies are shown in Supplementary Table 1. Secondary antibodies were horseradish peroxidase-coupled goat anti-mouse, anti-rabbit and anti-rat Igs (GE Healthcare), and Alexa fluorophore (350, 488 and 568) coupled goat anti-mouse and anti-rabbit IgGs (Invitrogen). AR-A014418 and CT99021 were from Abcam and Cayman respectively.

Stoica R., De Vos K.J., Paillusson S., Mueller S., Sancho R.M., Lau K.F., Vizcay-Barrena G., Lin W.L., Xu Y.F., Lewis J., Dickson D.W., Petrucelli L., Mitchell J.C., Shaw C.E, & Miller C.C. (2014). ER–mitochondria associations are regulated by the VAPB–PTPIP51 interaction and are disrupted by ALS/FTD-associated TDP-43. Nature Communications, 5, 3996.

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Antibodies for VAPB and PTPIP51 Proteins

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Rat and rabbit antibodies to VAPB and PTPIP51 have been described previously and were generated by immunization with GST‐VAPB(1–220) and GST‐PTPIP51(36–470) 5. Rabbit PTPIP51 antibody (FAM82A2) was from Atlas Antibodies. Rabbit anti‐haemagglutinin (HA), mouse anti‐α‐tubulin (DM1A) and rabbit anti‐mitofusin‐2 were from Sigma. Mouse anti‐myc (9B11) and rabbit anti‐glyceraldehyde 3‐phosphate dehydrogenase (GAPDH) were from Cell Signaling. Rabbit anti‐FUS (NB100‐565) was from Novus Biologicals. Rabbit anti‐TOM20 was from Santa Cruz Biotechnology and mouse anti‐PDI (RL77) was from Affinity Bioreagents. Antibodies to total and ser9 phosphorylated (inactive) GSK‐3β were from BD Transduction Labs (mouse 610201) and Cell Signalling (rabbit 9336), respectively. Rabbit anti‐GFP (Ab290) was from Abcam. GSK‐3β inhibitors AR‐A014418 and CT99021 were from Abcam and Cayman, respectively, and made up as 1 mM or 100 μM stocks in DMSO; KCN was from Sigma and made up as a 1 M stock in water.

Stoica R., Paillusson S., Gomez‐Suaga P., Mitchell J.C., Lau D.H., Gray E.H., Sancho R.M., Vizcay‐Barrena G., De Vos K.J., Shaw C.E., Hanger D.P., Noble W, & Miller C.C. (2016). ALS/FTD‐associated FUS activates GSK‐3β to disrupt the VAPB–PTPIP51 interaction and ER–mitochondria associations. EMBO Reports, 17(9), 1326-1342.

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