V7 plate

Manufactured by Agilent Technologies

Sourced in United States

The V7 plate is a laboratory equipment designed for various experimental and analytical applications. It provides a versatile platform for performing various procedures, such as sample preparation, incubation, and reagent dispensing. The V7 plate features a durable construction and a standardized layout, ensuring consistent and reliable results. However, a more detailed description of its core function and intended use would require additional information that I do not have access to at this time.

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Lab products found in correlation

Xf24 flux analyzer, by Agilent Technologies (1 mentions) Mitochondrial isolation kit, by Merck Group (1 mentions) Seahorse xf extracellular flux analyzer, by Agilent Technologies (1 mentions)

Spelling variants of this product

XF24 V7 cell culture plates (2 citations) V7 Seahorse tissue culture plates (2 citations) V7 24 well plates (2 citations)

2 protocols using v7 plate

Mitochondrial Respiration Profiling

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Mitochondrial oximetry was performed as previously described (Koczor, 2013b ). Briefly, mitochondria were isolated from mouse hearts immediately following sacrifice using differential centrifugation and a commercial mitochondrial isolation kit (Sigma Aldrich). An aliquot was quantitated for protein using the Bradford assay, and 5µg of protein was placed into a V7 plate (Seahorse Bioscience, Billerica, MA) and centrifuged at 3,400XG for 20 minutes at 4°C. Following centrifugation, pyruvate and malate were added to each well, and the mitochondrial respiration was analyzed in an XF24 flux analyzer (Seahorse Bioscience) using the manufacturer’s protocol. Basal respiration (oxygen consumption rate of the mitochondria immediately following equilibration), State III respiration, State IV_o respiration, and respiratory control ratio (RCR) were quantitated. Oximetric results are provided as pmol O₂/min/µg protein.

Koczor C.A., Fields E., Jedrzejczak M.J., Jiao Z., Ludaway T., Russ R., Shang J., Torres R.A, & Lewis W. (2015). Methamphetamine and HIV-Tat Alter Murine Cardiac DNA Methylation and Gene Expression. Toxicology and applied pharmacology, 288(3), 409-419.

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Mitochondrial Function Assessment in Islets

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OCR was determined using the Seahorse XF Extracellular Flux Analyzer (Seahorse Bioscience, North Billerica, MA, USA). After an overnight recovery, isolated islets from WT and HIP rats were seeded (25–50 islets per well) into the V7 plate (Seahorse Bioscience, North Billerica, MA, USA). To assess mitochondrial function, OCR was measured at the basal state and after stimulation with 20 mM glucose and sequential injection of oligomycin (ATP synthase inhibitor), carbonyl cyanide-p trifluoromethoxyphenylhydrazone (FCCP; uncoupler), and rotenone (complex I inhibitor).

Montemurro C., Nomoto H., Pei L., Parekh V.S., Vongbunyong K.E., Vadrevu S., Gurlo T., Butler A.E., Subramaniam R., Ritou E., Shirihai O.S., Satin L.S., Butler P.C, & Tudzarova S. (2019). IAPP toxicity activates HIF1α/PFKFB3 signaling delaying β-cell loss at the expense of β-cell function. Nature Communications, 10, 2679.

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