Cinnamic acid and other chemicals were obtained from Aladdin (Shanghai, China). A DNA gel extraction kit, plasmid purification kit, Primer STAR Max and DNA marker were obtained from TAKARA (Japan). Protein markers, restriction endonucleases and T4 DNA ligase were obtained from Thermo Fisher Scientific (USA). M9 Minimal Salts (M9 buffer) were obtained from Sangon Biotech (Shanghai, China). E. coli RARE(DE3) was purchased from Addgene (USA).
Plasmid purification kit
Manufactured by Takara Bio
Sourced in Japan
The Plasmid Purification Kit is a laboratory tool designed to isolate and purify plasmid DNA from bacterial cultures. The kit provides a reliable and efficient method for the extraction of plasmid DNA, which is commonly used in various molecular biology applications.
Automatically generated - may contain errors
Lab products found in correlation
Protein marker, by Thermo Fisher Scientific (2 mentions)
Dna gel extraction kit, by Takara Bio (2 mentions)
T4 dna ligase, by Thermo Fisher Scientific (2 mentions)
Dna marker, by Takara Bio (2 mentions)
Primer star max, by Takara Bio (2 mentions)
M9 minimal salts m9 buffer, by Sangon (2 mentions)
Restriction endonuclease, by Thermo Fisher Scientific (1 mentions)
Lsm 710 confocal microscope, by Zeiss (1 mentions)
E coli bl21 de3 competent cells, by Vazyme (1 mentions)
Bca protein concentration determination kit, by Solarbio (1 mentions)
Dna purification kit, by Takara Bio (1 mentions)
Isopropyl β d thiogalactoside, by Sangon (1 mentions)
Taq master mix, by Novoprotein (1 mentions)
Plasmid mini preparation kit, by Takara Bio (1 mentions)
Ampicillin, by Sangon (1 mentions)
4 protocols using plasmid purification kit
1
Molecular Cloning and Expression
2
Subcellular Localization of ZmREM1.3
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The ZmREM1.3 coding sequence in P178 plants was amplified using gene‐specific primers (Table S1 ) and cloned into the pCAMBIA1304 binary vector so that its expression was under the control of the cauliflower mosaic virus (CaMV) 35S promoter. The CaMV35S‐ZmREM1.3‐GFP and CaMV35S‐GFP constructs were firstly purified using plasmid purification kit (TaKaRa, Dalian, China), then transiently expressed in maize protoplasts, as previously described (Cao et al., 2014 ). After 16 h incubation at 25 °C, in the dark, GFP signal was observed and photographed using the LSM710 confocal microscope (Zeiss, Germany).
3
Bacterial Protein Expression Protocol
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2-FMA and other chemicals were obtained from Aladdin (Shanghai, China). A DNA gel extraction kit, plasmid purification kit, Primer STAR Max and DNA marker were obtained from TAKARA (Japan). Protein markers and T4 DNA ligase were obtained from Thermo Fisher Scientific (United States). M9 Minimal Salts (M9 buffer) were obtained from Sangon Biotech (Shanghai, China). E. coli BL21 (DE3) competent cells were purchased from Vazyme (Nanjing, China).
4
Enzymatic Synthesis and Analysis
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Phenylpyruvate, p-hydroxyphenylpyruvate and nicotinamide adenine dinucleotide (NADH) were purchased from Aladdin (Shanghai, China), benzoyl formic acid was purchased from Urchem (Shanghai, China), isopropyl-β-D-thiogalactoside (IPTG) and ampicillin were purchased from Sangon Biotech (Shanghai, China). 3,4dihydroxyphenylpyruvate was synthesized according to the method described in the literature (Bai et al., 2014) .
FastPfu PCR SuperMix was purchased from Miozyme (Shanghai, China), Taq Master Mix was purchased from Novoprotein (Suzhou, China), and ABclonal MultiF Seamless Assembly was purchased from abclonal (Wuhan, China). BCA protein concentration determination kit was obtained from Solarbio (Beijing, China). Restriction endonucleases (SacⅠ, BamHI), QuickCut TM DpnⅠ, plasmid mini preparation kit, Plasmid Purification Kit and DNA purification kit were purchased from TaKaRa (Otsu, Japan). Color pre-stained standard protein Marker was purchased from Adamas (Shanghai, China). All of the above chemical reagents are analytical grade.
FastPfu PCR SuperMix was purchased from Miozyme (Shanghai, China), Taq Master Mix was purchased from Novoprotein (Suzhou, China), and ABclonal MultiF Seamless Assembly was purchased from abclonal (Wuhan, China). BCA protein concentration determination kit was obtained from Solarbio (Beijing, China). Restriction endonucleases (SacⅠ, BamHI), QuickCut TM DpnⅠ, plasmid mini preparation kit, Plasmid Purification Kit and DNA purification kit were purchased from TaKaRa (Otsu, Japan). Color pre-stained standard protein Marker was purchased from Adamas (Shanghai, China). All of the above chemical reagents are analytical grade.
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