All culture media were from Life Technologies and were supplemented with 10% fetal calf serum, 2 mM glutamine, 125 U/ml penicillin and 125 μg/ml streptomycin. All cells were grown at 37°C in a humidified atmosphere with 5% CO2. U2OS and derivatives cells, GM00637 (from healthy individual) and GM05849 (from Ataxia-telangiectasia patient) SV40-transformed human fibroblasts (Coriell Institute, Camden, NJ, USA) were grown in Dulbecco's modified Eagle's medium. HCT116 XLF-defective (Horizon Discovery Ltd, Cambridge, UK) and parental HCT116 cell lines were maintained in RPMI 1640 medium. NALM6, N114P2 and derivatives cells were grown in suspension in RPMI 1640 medium.
Gm05849
Manufactured by Coriell Institute for Medical Research
Sourced in United States
GM05849 is a cell line derived from human skin fibroblasts. The core function of this product is to provide a source of human cells for research purposes.
Automatically generated - may contain errors
Lab products found in correlation
Gm00637, by Coriell Institute for Medical Research (2 mentions)
Bortezomib, by MedChemExpress (1 mentions)
Ibrutinib, by MedChemExpress (1 mentions)
Spironolactone, by Selleck Chemicals (1 mentions)
Thp 1, by American Type Culture Collection (1 mentions)
Aml 193, by American Type Culture Collection (1 mentions)
Rpmi8226, by American Type Culture Collection (1 mentions)
Su dhl 10, by American Type Culture Collection (1 mentions)
Su dhl 4, by American Type Culture Collection (1 mentions)
Su dhl 6, by American Type Culture Collection (1 mentions)
Jeko 1, by American Type Culture Collection (1 mentions)
Rec 1, by American Type Culture Collection (1 mentions)
Maver 1, by American Type Culture Collection (1 mentions)
Z 138, by American Type Culture Collection (1 mentions)
Sup b15, by American Type Culture Collection (1 mentions)
Nci h929, by American Type Culture Collection (1 mentions)
Jvm 2, by American Type Culture Collection (1 mentions)
Ku812, by American Type Culture Collection (1 mentions)
Kasumi 6, by American Type Culture Collection (1 mentions)
2 protocols using gm05849
1
Cell Culture Conditions for Diverse Cell Lines
2
Cell Lines, Small Molecules, and Formulations
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The following cell lines were purchased from the American Type Culture Collection (ATCC): K-562R, THP-1, KASUMI-6, AML-193, SUP-B15, KU-812, U-937, CA46, SU-DHL-6, REH, NCI-H929, JVM-2, JEKO-1, MAVER-1, MINO, REC-1, Z-138, RPMI8226, SU-DHL-4, and SU-DHL-10. OCI-LY1 was purchased from the German Collection of Microorganisms and Cell Cultures GmbH (DSMZ). DOHH-2, OCI-LY2, OCI-LY-8, TMD8, HAP1, AA8, UV5, UV20, UV21, and EM9 were available at and authenticated by our contract research organizations. Human fibroblast cells GM00637 and GM05849 were purchased from Coriell Institute for Medical Research. All the cell lines were cultured in medium according to the vendors’ recommendation in cell culture incubators set at 37°C with 5% CO2. LP-284 was provided by Lantern Pharma Inc., and stored at −80°C. LP-284 was formulated from powder by dissolving in 5% EtOH and 95% saline (in vivo studies) or DMSO (in vitro studies). Bortezomib was purchased from MedChemExpress (Cat. HY-10227) and formulated with 1% DMSO and 99% Saline. Ibrutinib was purchased from MedChemExpress (Cat. HY-10997) and formulated with 10% DMSO, 40% PEG 300, 5% Tween 80, and 45% saline. Spironolactone was purchased from Selleckchem (Catalog No.S4054) and formulated with 100% DMSO.
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