Ata 27 arrayer

The Institutional Review Board at The University of Chicago (Chicago, IL) approved all of the human subjects research performed in this study. Written informed consent was obtained from all patients involved in the study. All methods were carried out in accordance with the respective approved protocol. Duplicate samples from fifty-seven MM tumors including 36 epithelioid, and 20 biphasic were processed into a tissue microarray (TMA) under an institutional review board approved protocol as previously described67 (link). Patient and tumor characteristics are included in Table 2. Control tissues included 15 benign hyperplastic mesothelial samples in duplicate. The TMA was built using the ATA-27 Arrayer from Beecher Instruments. In brief, tissue cores (1-mm punch) from biopsied tissue samples were precisely organized into a grid and embedded in paraffin. The paraffin block was cut and the TMA was processed for H&E staining. Pathologic diagnosis in these cases was reviewed by at least two experienced pathologists. The H&E stained TMA was scanned using a 3D- Histech Pannoramic SCAN whole slide scanner.