Other oligonucleotides

3' adapter (RA3-A2N) was obtained from Takara Biomedical Technology (Beijing, China); 5' adapter (SR5F) was obtained from Sangon Biotech (Shanghai, China). Other oligonucleotides were obtained from Sangon Biotech. All oligonucleotides used in this study are described in Supplementary Table S3.

Acrylamide, ammonium persulfate (APS), and H₂O₂ (30%) were obtained from Sinopharm Chemical Reagent (Shanghai, China). 3,3′,5,5′-tetramethylbenzidine (TMB), N,N,N′,N′-tetramethylethylenediamine (TEMED), tris(hydroxymethyl)aminomethane (Tris), Dulbecco’s modified Eagle’s medium (DMEM), fetal bovine serum (FBS), and penicillin-streptomycin were obtained from Sigma-Aldrich (St. Louis, MO, USA). HRP was purchased from J&K Scientific Ltd. (Beijing, China). TRIzol solution, acrylic-DNA and all other oligonucleotides used in this study (Supplementary Table S1) were synthesized by Sangon Biotech Co., Ltd. (Shanghai, China). Other reagents were purchased from Damao Chemical Reagent Factory (Tianjin, China). HeLa cells were obtained from Sangon Biotech Co., Ltd.

Oligonucleotides with a length > 100 bp were purchased from GenScript, and other Oligonucleotides were purchased from Sangon Biotech (see sequences used in this study). dNTPs were purchased from Solarbio. Klenow fragment DNA polymerase I was purchased from ABclonal Biotech Co., Ltd. OneTaq DNA polymerase, Deep vent DNA polymerase, glycosylase (UDG), apurinic/apyrimidinic Endonuclease 1 (APE1), as well as T4-DNA ligase, were purchased from New England Biolabs. 2 × UItraSYBR Mixture was purchased from CWBIO Biotech co., Ltd. pBLUE-T Fast Cloning Kits and BL 21 (DE3) Electrocompetent cells were purchased from Zoman Biotechnology Co., Ltd. dNaMTP, dTPT3TP, and dTPT3^PA were synthesized as reported (8 (link),24 (link)). NMR spectra were performed on AVANCE NanoBay (400 MHz). HRMS or MS were performed on Bruker compact Ultra-high-resolution electro-spray time-of-flight mass spectrometry and Bruker Autoflex speed MALDLTOF/TOF spectrometry, respectively.