Methoxypolyethyleneglycol mw 2000 distearylphosphatidylethanolamine mpeg2000 dspe

Hydrogenated soya phosphatidylcholine (HSPC) and Methoxypolyethyleneglycol (Mw 2000)-distearylphosphatidylethanolamine (mPEG2000-DSPE) were purchased from Lipoid (Ludwigshafen, Germany). Maleimide-PEG2000 distearoylphosphatidylethanolamine (Mal-PEG2000-DSPE) and 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine-N-(lissamine rhodamine B sulfonyl) (Liss Rhod, PE) were purchased from Avanti Polar Lipids (Alabaster, AL, USA). Then, 3,3′-Dioctadecyloxacarbocyanine perchlorate (DiO); 1,1′-Dioctadecyl-3,3,3′,3′-Tetramethylindodicarbocyanine; 4-Chlorobenzenesulfonate Salt (DiD); and LysoTracker Red were purchased from Thermo Fisher Scientific (Waltham, MA, USA). Ammonium sulfate, Cholesterol, and doxorubicin hydrochloride (Dox) were purchased from Sigma-Aldrich (St Louis, MO, USA). RGDf[N-met]C was synthesized by Ansynth Service B.V. (Roosendaal, The Netherlands). A TAT peptide analog equipped with 3G as a spacer and a C for conjugation (CGGG-RKKRRQRRRGYG) was synthesized by Peptron Inc. (Daejeon, South Korea). All other solvents and reagents were used as chemical grade.