After treatment, the cells were fixed with 4.0% PFA for 30 min at room temperature, washed three times, permeabilized with 0.5% Triton X-100 for 5 min at room temperature, incubated in 5% bovine serum albumin for 1 h, and then incubated with antibodies against CD44 (Abcam) at 1:400 overnight. After washing with PBS, the cells were incubated with Alexa Fluor 488 conjugated secondary antibody (Thermo Fisher Scientific). Finally, the nuclei were stained with DAPI (1:1000, Beyotime). The imaging was captured by confocal microscopy.
Antibodies against cd44
Manufactured by Abcam
Sourced in United States
Antibodies against CD44 are laboratory reagents used for the detection and study of the CD44 cell surface glycoprotein. CD44 is a transmembrane receptor involved in cell-cell interactions, cell adhesion, and migration. These antibodies can be used in various immunological techniques to identify and analyze CD44-expressing cells.
Automatically generated - may contain errors
Lab products found in correlation
Alexa fluor 488 conjugated secondary antibody, by Thermo Fisher Scientific (1 mentions)
4 6 diamidino 2 phenylindole (dapi), by Beyotime (1 mentions)
Epigallocatechin 3 gallate, by Meilun (1 mentions)
β actin, by Abcam (1 mentions)
Caspase 3, by Abcam (1 mentions)
Doxorubicin, by Meilun (1 mentions)
2 protocols using antibodies against cd44
1
CD44 Immunofluorescence in Fixed Cells
2
Hyaluronic Acid-Based Nanomedicine Synthesis
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Hyaluronic acid (HA, 97%) (MW ≈ 9000 Da) was purchased from Shandong Freda Biopharmaceutical Co., Ltd. Cysteamine dihydrochloride (>97%), 4-(4,6-dimethoxy-1,3,5-triazin-2-yl)-4-methylmorpholinium chloride (DMTMM, 97%), and glutathione (GSH, 98%) were purchased from Aladdin (Shanghai, China). Iron chloride hexahydrate (FeCl3·6H2O, 99%) was purchased from Sigma-Aldrich. Epigallocatechin-3-gallate (EGCG, 95%) and Doxorubicin (DOX, >98%) were purchased from Meilun Biotechnology Co., Ltd. Annexin V-fluorescein isothiocyanate (FITC)/7-aminoactinomycin D (7AAD), 4′,6-diamidino-2-phenylindole (DAPI), and lysosomal probes were purchased from Beyotime Biotechnology (Shanghai). Antibodies against CD44, CD31, Ki67, β-actin, and caspase 3 were purchased from Abcam (Massachusetts, USA).
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