Mouse crp elisa kit

Plasma samples were thawed, vortexed, and centrifuged for 10 min at 1000 rpm. C-Reactive Protein (CRP) plasma levels were measured using the Mouse CRP ELISA Kit (Invitrogen by Thermo Fisher Scientific, Waltham, MA, USA) according to the manufacturer’s instructions, diluting all samples 2000-fold. Serum amyloid antigen (SAA) plasmatic concentrations were determined using the Phase SAA Murine Assay Kit (Tridelta Development Ltd., Maynooth, Ireland), diluting the samples between 50–100-fold for the TNBS-colitis and 500–8000-fold for DSS-colitis. All samples were analyzed in duplicates, and OD was measured in the Varioskan microplate reader (Thermo Fisher Scientific, Waltham, MA, USA). Standard curves were generated. For the CRP quantification, a four-parameter algorithm in the MyCurveFit Add-in in Microsoft Excel 2016 software (version R2 v1.0102.823, MyAssays Ltd, Brighton, United Kingdom).

The plasma levels of CRP were measured with the Mouse CRP ELISA Kit (Invitrogen, Thermo Fisher Scientific).
TNFα was measured with the Mouse TNF alpha uncoated ELISA kit (Invitrogen, Thermo Fisher Scientific) in plasma and brain tissue samples.
Global DNA methylation was determined with the MethylFlash Methylated DNA 5-mC Quantification kit (Colorimetric) (EpiGentek, Farmingdale, New York, NY, USA). Global DNA hydroxymethylation was determined with the MethylFlash Global Hydroxymethylation (5-hmC) ELISA Easy Kit (Colorimetric) (EpiGentek). DNA methylation and hydroxymethylation were measured in brain samples.
The Human Aβ₄₂ ELISA Kit (Invitrogen, Thermo Fisher Scientific) was used to quantify Aβ₄₂ in 5XFAD mouse brains.
Sandwich ELISA assays were performed according to the specific manufacturer′s instructions. Absorbances were measured in a spectrophotometer Multiskan SkyHigh (Thermo Fisher Scientific) and the corresponding concentrations were calculated from standard curves. The samples were tested in duplicate.

We utilized the ELISA kit from Raybiotech (Norcross, GA, USA) to assess the concentrations of serum TNF-α and IL-6. Duplicate analyses were performed for each sample. The intra-assay variance for TNF-α and IL-6 was 6.7% and 5.5%, respectively. Furthermore, serum CRP levels were assessed utilizing an Invitrogen (Thermo Fisher Scientific, USA) mouse CRP ELISA kit. For CRP, the intra-assay variation was measured at 3.4%. To mitigate inter-assay variance, all samples were processed simultaneously.