Primocin

Manufactured by Atlanta Biologicals

Primocin is a broad-spectrum antibiotic solution for use in cell culture applications. It is a combination of two antibiotics, Primocin M and Primocin V, designed to provide effective control of microbial contamination in cell culture systems.

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Lab products found in correlation

Fetal bovine serum (fbs), by Atlanta Biologicals (2 mentions) Human insulin, by Merck Group (1 mentions) Horse serum, by Atlanta Biologicals (1 mentions) Primocin antibiotic, by InvivoGen (1 mentions) Dulbecco s phosphate buffered saline dpbs, by Thermo Fisher Scientific (1 mentions) Six well plate, by Corning (1 mentions) Evos fl auto microscope, by Thermo Fisher Scientific (1 mentions) Carl lsm700 confocal microscope, by Zeiss (1 mentions)

2 protocols using primocin

Cell Culture Conditions for Cancer Research

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T47D, MCF7, ZR75 and JAR cells were maintained in RPMI 1640 medium supplemented with 10% fetal bovine serum (FBS; Atlanta Biologicals, Lawrenceville, GA) and 0.01% Primocin antibiotic (Invivogen, San Diego, CA). T47D cell medium was supplemented with 5 ug/ml of human insulin (Sigma-Aldrich). MB231 and MB468 cells were maintained in DMEM with 1 g/L glucose supplemented with 10% FBS and 0.01% Primocin. MCF10a and HME cells were maintained in DMEM F:12 supplemented with 5% horse serum (Atlanta Biologicals) or 0.4% bovine pituitary extract respectively, and 5 ug/ml human insulin, 0.5 ug/ml hydrocortisone, 20 ng/ml human epidermal growth factor and 0.01% Primocin. BeWo cells were maintained in DMEM F:12 supplemented with 10% FBS and 0.01% Primocin. Hek293 cells were maintained in DMEM with 4.5 g/L glucose, 10% FBS and 0.01% Primocin.

Tuttle T.R., Hugo E.R., Tong W.S, & Ben-Jonathan N. (2014). Placental Lactogen Is Expressed but Is Not Translated into Protein in Breast Cancer. PLoS ONE, 9(1), e87325.

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Rat Aortic Smooth Muscle Cell Culture

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Rat SMCs (A7r5) were purchased (ATCC; CRL-1444; Manassas, VA) and grown in Dulbecco’s modified Eagle’s medium (DMEM) containing 0.2% Primocin and 10% fetal bovine serum (Atlanta Biologicals; Flowery Branch, GA) (referred to as “complete media”) up to passage 15. Cells were maintained at 37°C and 5% CO₂ in a humidified incubator (ShelLab; Cornelius, OR) and serially passaged 1:2 in tissue culture-treated 75 cm² flasks (USA Scientific; Ocala, FL). For trypsinization, conditioned media was aspirated, flasks were washed with 1× Dulbecco’s phosphate buffered saline (DPBS; Thermo Fisher Scientific; Waltham, MA) then cells were incubated for 5 min in 0.25% Trypsin/2.21 mM EDTA in Hank’s Balanced Salt Solution (HBSS) without sodium bicarbonate, calcium, or magnesium (Corning; Tewksbury, MA). Cell suspensions (supplemented with complete growth medium) were seeded in appropriate vessels: 60 mm petri dishes, six-well plates (tissue culture (TC)-treated; Corning), and ibidi 6.4 microchannel slides (glass or TC-treated bottoms; ibidi USA; Madison, WI) and allowed to attach/spread for specified times. Images were captured using a Leica DMI4000B inverted fluorescent microscope and CoolSnap HQ2 camera, EVOS FL Auto microscope (Life Technologies), or Carl Zeiss LSM 700 confocal microscope (Carl Zeiss, Germany).

Holt A.W., Howard W.E., Ables E.T., George S.M., Kukoly C.A., Rabidou J.E., Francisco J.T., Chukwu A.N, & Tulis D.A. (2016). Making the Cut: Innovative Methods for Optimizing Perfusion-Based Migration Assays. Cytometry. Part A : the journal of the International Society for Analytical Cytology, 91(3), 270-280.

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