Great escape seap chemiluminescent kit

Manufactured by Takara Bio

Sourced in Japan

The Great EscAPe SEAP Chemiluminescent kit is a laboratory assay designed to detect and quantify secreted alkaline phosphatase (SEAP) activity in cell culture samples. The kit utilizes a chemiluminescent substrate to measure SEAP levels, providing a sensitive and reliable method for monitoring gene expression or cell signaling pathways.

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Lab products found in correlation

Primestar gxl polymerase, by Takara Bio (2 mentions) Skim milk, by Fujifilm (1 mentions) Ecl prime western blotting detection system, by GE Healthcare (1 mentions) Pebmultipuro, by Fujifilm (1 mentions) Flagellin, by Adipogen (1 mentions) Hygromycin b gold, by InvivoGen (1 mentions) Pebmulti neo, by Fujifilm (1 mentions) Blasticidine, by InvivoGen (1 mentions) Normocin, by InvivoGen (1 mentions) Pnifty2 seap, by InvivoGen (1 mentions) Quanti blue, by InvivoGen (1 mentions) Lipopolysaccharides (lps), by Merck Group (1 mentions)

2 protocols using great escape seap chemiluminescent kit

Screening Marine Bacteria and Synthetic Compounds

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PrimeStar GXL polymerase, Great EscAPe SEAP Chemiluminescent kit and DNA ligation kit, Mighty Mix was obtained from TaKaRa Bio, Shiga, Japan. pEB-Multi-Puro, skim milk and G418 were obtained from Wako Pure Chemical Industries, Ltd., Chuo-ku, Osaka, Japan. Four primers as shown in Supplement Information were obtained from Integrated DNA Technologies, Inc., Skokie, Illinois, IL, USA. Puromycin and fetal bovine serum (FBS) were obtained from Sigma Aldrich, St. Louis, MO, USA. Anti-human IL-18 polyclonal antibody (#PM014) and anti-GAPDH monoclonal antibody (#M171-3), and recombinant mature form human (#B001-5) and mouse (#B002-5) IL-18 (generated from E. coli) were obtained from MBL CO., LTD., Nagoya, Japan. ECL prime Western Blotting Detection System were obtained from GE Healthcare, Uppsala, Sweden. Extracts from marine bacteria and synthetic compound library were kindly gifted from Prof. Yamada and Prof. Ishihara, respectively. Complementary DNA (cDNA) derived from human peripheral blood mononuclear cells (PBMCs) was kindly provided by Dr. Isagawa.

Kurata R., Shimizu K., Cui X., Harada M., Isagawa T., Semba H., Ishihara J., Yamada K., Nagai J., Yoshida Y., Takeda N., Maemura K, & Yonezawa T. (2020). Novel Reporter System Monitoring IL-18 Specific Signaling Can Be Applied to High-Throughput Screening. Marine Drugs, 18(1), 60.

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High-throughput screening of natural products against TLR4 signaling

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PrimeStar GXL polymerase, Great EscAPe SEAP Chemiluminescent kit and In-Fusion HD cloning kit reagent were obtained from TaKaRa Bio, Japan. pEB-Multi-Neo and G418 were obtained from Wako, Japan. Normocin, Blasticidine, Hygromycin B Gold, QUANTI-Blue, HEK293T-TLR4-MD2-CD14, pNiFty2-SEAP and pNiFty2-56K-SEAP were obtained from InvivoGen, San Diego, CA, USA. LPS were obtained from Sigma-Aldrich, St. Louis, MO, USA. Recombinant human IFN-beta was obtained from Peprotech, Rocky Hill, NJ, USA. Recombinant human CIRP and Flagellin (high purity) were obtained from Adipogen life sciences, San Diego, CA, USA. Mouse S100A13 and PTMA were generated from E coli and then, were subjected to endotoxin removal resin. In both of them the endotoxin concentration was below 1EU per microgram protein. Natural products library was kindly gifted from Dr. Yamada and Prof. Takeda.

Kurata R., Kumagai A., Cui X., Harada M., Nagai J., Yoshida Y., Ozaki K.I., Tanaka Y, & Yonezawa T. (2018). Establishment of Novel Reporter Cells Stably Maintaining Transcription Factor-driven Human Secreted Alkaline Phosphatase Expression. Current pharmaceutical biotechnology, 19(3).

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