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Pmys ires gfp

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The PMYs-IRES-GFP is a mammalian expression vector that contains an internal ribosome entry site (IRES) and the green fluorescent protein (GFP) reporter gene. The IRES sequence allows for the translation of the GFP gene from the same mRNA transcript as the gene of interest, enabling the visualization of transfected cells.

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Lab products found in correlation

Luciferase reporter assay, by Promega (1 mentions) Pcdna3 expression vector, by Thermo Fisher Scientific (1 mentions) Fugene 6, by Promega (1 mentions)

Spelling variants of this product

PMY-IRES-GFP retroviral vector (3 citations) PMYs-IRES-GFP Retroviral Vector (2 citations)

2 protocols using pmys ires gfp

1

Plasmid Characterization and Cloning

Cited 1 time
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The following plasmids were previously described: pGa981/6, pcDNA3-Flag-SPOC, pcDNA3-RBP;33 (link) pcDNA3-Flag-SPOC pcDNA3-F3, pcDNA3-F3-AML1-ETO, pcDNA3-AML1-ETO(tr)-GFP;31 (link) pcDNA3-Flag-SPOC (Y3602A), pcDNA3-Flag-SPOC (K3516A), pcDNA3-Flag-SPOC (R3552A/R3554A), pcDNA3-RBP-SPOC pcDNA3-RBP-SPOC (Y3602A), pcDNA3-RBP-SPOC (K3516A), pcDNA3-RBP-SPOC (R3552A/R3554A).34 (link) The plasmid pMYs-IRES-GFP was commercially achieved from Cell Biolabs, San Diego, CA, USA. Sequence information of all additional vectors used in this study is available on request.
Thiel V.N., Giaimo B.D., Schwarz P., Soller K., Vas V., Bartkuhn M., Blätte T.J., Döhner K., Bullinger L., Borggrefe T., Geiger H, & Oswald F. (2017). Heterodimerization of AML1/ETO with CBFβ is required for leukemogenesis but not for myeloproliferation. Leukemia, 31(11), 2491-2502.
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2

Hoxa2 Promoter Regulation in HEK293T Cells

Cited 1 time
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HEK293T cells were transfected using Fugene 6 (Promega), with a total of 1 μg DNA, containing 550 ng pGL3-Hoxa2 promoter (Hoxa2 promoter from −220 to +1 cloned in pGL3 [Promega]) and 150 ng of each Hoxa2, Meis1, and Pbx1a in pCDNA3 expression vector or pCDNA3 control (Life Technologies). Cells were harvested 24 hr after transfection for luciferase reporter assays (Promega). IBAs were dissociated into single cells and infected using supernatants from Ecotropic-Phoenix packaging cells, transfected with pMYs-IRES-GFP (Cell Biolabs) or pMYs-Hoxa2-IRES-GFP (Anderson et al., 2013 (link)). The infection efficiency, evaluated by fluorescence-activated cell sorting, was 70%. Cells were cultured for 72 hr and their chromatin extracted for chromatin immunoprecipitation (ChIP).
Amin S., Donaldson I.J., Zannino D.A., Hensman J., Rattray M., Losa M., Spitz F., Ladam F., Sagerström C, & Bobola N. (2015). Hoxa2 Selectively Enhances Meis Binding to Change a Branchial Arch Ground State. Developmental Cell, 32(3), 265-277.
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