Log In Sign up
The largest database of trusted experimental protocols

Cf300 cu 50 grid

Manufactured by Electron Microscopy Sciences
Visit Supplier

The CF300-Cu-50 grid is a laboratory equipment designed for use with electron microscopes. It provides a substrate for holding and supporting samples during microscopic examination. The grid is made of copper and has a mesh size of 300 lines per inch, with a 50-micron bar width. This product is intended to facilitate the preparation and observation of specimens in electron microscopy applications.

Automatically generated - may contain errors

Lab products found in correlation

Tecnai 12 120 kv biotwin spirit microscope, by Thermo Fisher Scientific (1 mentions) Tecnai 12 120 kv biotwin spirit tem, by Thermo Fisher Scientific (1 mentions)

Close spelling variants of this product

CF300-CU (38 citations) CF300-Cu grid (3 citations) CF300-CU-50 (2 citations)

2 protocols using cf300 cu 50 grid

1

SARS-CoV-2 Spike Protein Visualization

Check if the same lab product or an alternative is used in the 5 most similar protocols
Four microliters of SARS-CoV S protein (0.05 mg/ml) was applied onto a freshly glow discharged (1 min at 10 mA) CF300-Cu-50 grid (Electron Microscopy Sciences), incubated for 1 min, and manually blotted. Four microliters of 3% uranyl acetate was applied onto the same grid and incubated for 1 min before the solution was blotted off. Images were acquired at a nominal magnification of ×49,000 on a FEI Tecnai 12 120-kV BioTwin Spirit microscope.
Toelzer C., Gupta K., Yadav S.K., Hodgson L., Williamson M.K., Buzas D., Borucu U., Powers K., Stenner R., Vasileiou K., Garzoni F., Fitzgerald D., Payré C., Gautam G., Lambeau G., Davidson A.D., Verkade P., Frank M., Berger I, & Schaffitzel C. (2022). The free fatty acid–binding pocket is a conserved hallmark in pathogenic β-coronavirus spike proteins from SARS-CoV to Omicron. Science Advances, 8(47), eadc9179.
+ Open protocol
+ Expand
2

SARS-CoV-2 Spike Protein Imaging

Check if the same lab product or an alternative is used in the 5 most similar protocols
In all, 4 µL of 0.05 mg/mL SARS-CoV-2 spike protein was applied onto a freshly glow discharged (1 min at 10 mA) CF300-Cu-50 grid (Electron Microscopy Sciences), incubated for 1 min, and manually blotted. In total, 4 µL of 3% uranyl acetate was applied onto the same grid and incubated for 1 min before the solution was blotted off. The grid was loaded onto a FEI Tecnai12 120 kV BioTwin Spirit TEM. Images were acquired at a nominal magnification of ×49,000.
Gupta K., Toelzer C., Williamson M.K., Shoemark D.K., Oliveira A.S., Matthews D.A., Almuqrin A., Staufer O., Yadav S.K., Borucu U., Garzoni F., Fitzgerald D., Spatz J., Mulholland A.J., Davidson A.D., Schaffitzel C, & Berger I. (2022). Structural insights in cell-type specific evolution of intra-host diversity by SARS-CoV-2. Nature Communications, 13, 222.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!