Cd3e apc

Manufactured by Thermo Fisher Scientific

CD3e-APC is a fluorescently-labeled antibody that binds to the CD3 epsilon chain, a component of the T cell receptor complex. It is used for the identification and enumeration of T cells in flow cytometry applications.

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APC-anti-mouse CD3e (7 citations) APC-anti-mouse CD3e (145-2C11, (4 citations) Anti-CD3e-APC (3 citations) Anti-human CD3E (SK7)-APC (2 citations)

2 protocols using cd3e apc

Characterizing Lymph Node Immune Cells

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To analyze immune cell populations in the draining lymph nodes, the cells were isolated by slitting the organs and pressing them through 100 µm mesh cups (Corning, # 52360) to generate single-cell suspensions. 2 × 10⁶ cells were stained with different combination of the following antibodies: CD11c-FITC (# 553801), CD4-PE (# 553730), CD27-PE (# 558754), NK1.1-PE-Cy7 (# 552878), 7AAD (# 559925) CD19-V450, (# 560375, CD11b-V450 (# 560455) all from BD Biosciences; CD3e-APC (# 17–0031) from eBioscience ; AnnexinV-FITC from Immunotools (# 31490013) and CD8a-FITC from Miltenyi (# 130–102–806). A minimum of 5 × 10⁵ events were detected per measurement. Flow cytometry was performed on a FACS Canto II (BD Biosciences, Heidelberg, Germany) and analyzed using FlowJo Software v7.6.5 (Treestar). The gating strategy is representatively depicted in Fig. S2.

Finkel P., Frey B., Mayer F., Bösl K., Werthmöller N., Mackensen A., Gaipl U.S, & Ullrich E. (2016). The dual role of NK cells in antitumor reactions triggered by ionizing radiation in combination with hyperthermia. Oncoimmunology, 5(6), e1101206.

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Leukemia Immunophenotyping by Flow Cytometry

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Bone marrow was harvested from mice seven days after leukemia transplantation and stained with CD3e-APC, CD4-PE-Cy7, CD8b-PE, B220-eFluor 450, and Fixable Viability Dye eFluor450 (eBioscience, San Diego, CA). Samples were analyzed on a Gallios561 flow cytometer. Leukemia cells cultured ex vivo were stained for MHCI-FITC, MHCII-FITC, PD-L2 FITC, CD40-FITC, CTLA-4-PE, CD80 PECy-7, CD86-FITC, CXCR4-Alexa Fluor 488 (eBioscience) or PD-L1 PE-Cy7 (Life Technologies, Frederick, MD) and analyzed on a Guava Easy Cyte Plus. FlowJo software (TreeStar) was used for data analysis.

Rabe J.L., Gardner L., Hunter R., Fonseca J.A., Dougan J., Gearheart C.M., Leibowitz M.S., Lee-Miller C., Baturin D., Fosmire S.P., Zelasko S.E., Jones C.L., Slansky J.E., Rupji M., Dwivedi B., Henry C.J, & Porter C.C. (2019). IL-12 abrogates calcineurin-dependent immune evasion during leukemia progression. Cancer research, 79(14), 3702-3713.

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