Tau r3

Genipin was purchased from MedChemExpress (Monmouth Junction, New Jersey, USA). Tau-R3 was obtained from ChinaPeptides (Shanghai, China). Heparin sodium salt was obtained from Aladdin (Shanghai, China). Thio avin T (ThT) was obtained from Sigma-Aldrich (St. Louis, MO, USA). Dulbecco's modi ed Eagle medium (DMEM), F12-DMEM, opti-MEM, neurobasal medium, B27 supplement, streptomycin, penicillin, L-glutamine and phosphate buffer solution (PBS) were purchased from Gibco (Grand Island, NY, USA). Fetal bovine serum (FBS) was supplied from Biological Industries (Kibbutz Beit Haemek, Israel). The cell counting kit (CCK)-8 and bicinchoninic acid (BCA) protein assay kit were provided by Beyotime (Jiangsu, China). Protease and phosphatase inhibitors were obtained from Bimake (Shanghai, China). The following antibodies were used in this study: anti-Tau, anti-phospho-T231, antiphospho-S396, anti-phospho-S404, anti-CDK5, anti-phospho-GSK-3β (Tyr 216 ), anti-LC3, anti-amyloid precursor protein (APP), anti-Aβ, anti-BACE1, and anti-β-actin (Abcam, Cambridge, UK); anti-p62, anti-Beclin-1, anti-SIRT1, anti-LKB1, anti-phospho-LKB1, anti-AMPK, anti-phospho-AMPK, anti-mTOR, antiphospho-mTOR, anti-p70S6K, anti-phospho-p70S6K, anti-PERK, anti-phospho-PERK, anti-eIF2a, and antiphospho-eIF2a (Cell Signaling Technology, Beverly, MA, USA).