Rabbit polyclonal affinity–purified anti-Cyclin Ba/b (acetyl-NRDLNIQESGPVKAVVNAC-amide; acetyl-CLEFLRRFSRVAEETIDPKEY-amide), anti-CDK1b (acety1-CPDFTKPSTLNVNTSLNEMDM-amide), anti-CDK1c (acetyl-MRKRNIDRPPTQDLNNYC–amide), ant-CDK1d (acety1-MIKAKSGTQDLSNYKRC-amide), and anti-lamin1 (acetyl-QSPISLPPLSGSTC-amide) were from 21st Century Biochemicals (Marlboro, MA). Other antibodies: anti-PSTAIR (Abcam, ab10345), anti-ATPB (Abcam, ab37922), anti-histone 3 (Abcam, ab10799), anti-H3-S28 (Abcam, ab10543), anti-GFP (AMS biotechnology, M30939-2). Secondary antibodies for immunofluorescence: goat anti-rabbit IgG (H+L) Alexa Fluor 568 (Thermofisher, A-11011); and for western blots: goat anti-rabbit IgG (H+L) superclonal™ secondary antibody, HRP (Thermofisher, A27033), Peroxidase-AffiniPure Goat Anti-Mouse IgG (H+L) (Jackson ImmunoResearch, 115-035-003).
Anti gfp
Manufactured by AMS Biotechnology
The Anti-GFP is a laboratory reagent designed to detect and quantify the presence of Green Fluorescent Protein (GFP) in biological samples. It functions as a highly specific antibody that binds to GFP, enabling researchers to analyze and track the expression of GFP-tagged proteins in their experiments.
Automatically generated - may contain errors
Lab products found in correlation
Anti histone 3, by Abcam (1 mentions)
Peroxidase affinipure goat anti mouse igg h l, by Jackson ImmunoResearch (1 mentions)
Anti atpb, by Abcam (1 mentions)
Anti pstair, by Abcam (1 mentions)
Alexa fluor 568 goat anti rabbit igg h l, by Thermo Fisher Scientific (1 mentions)
Alexa fluor 488, by Thermo Fisher Scientific (1 mentions)
Tp401, by AMS Biotechnology (1 mentions)
Anti acetylated tubulin, by Merck Group (1 mentions)
Anti huc d, by Thermo Fisher Scientific (1 mentions)
Anti ha, by Roche (1 mentions)
Pvdf membrane, by GE Healthcare (1 mentions)
Nbt bcip, by Roche (1 mentions)
4 protocols using anti gfp
1
Antibodies for Cell Cycle Analysis
2
Whole Mount Immunostaining Protocol
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Whole mount immunostaining was performed as previously described.42 (link) As primary antibodies, we used anti-GFP (AMS Biotechnology cat#TP401) at 1 : 1000. As secondary antibodies, we used Alexa Fluor 488 (A-11034) from Life Technologies.
3
Visualizing Gene Expression and Proteins
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Gene expression was visualised by in situ hybridization and proteins detected by immunohistochemistry as previously described.47,48 Antibodies used were anti-HuC/D (1 : 500, Invitrogen), anti-Isl1 (1 : 200, DSHB), anti-acetylated tubulin (1 : 200, Sigma), anti-GFP (1 : 500, AMS Biotechnology), anti-HA (1 : 300, Roche). Back labeling of axons was performed by applying DiI or DiD to muscles of fixed 5 dpf larvae using a sharpened tungsten needle. Fluorescent images were acquired using a Nikon C-1 Eclipse confocal microscope and processed using Photoshop (Adobe).
4
Western Blot Protein Detection
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Tissue was ground in liquid nitrogen and 4 × SDS buffer (Novex) was added and heated at 95°C for 5 min, cooled to room temperature for 10 min, samples were centrifuged 5 min at 15,682g. Supernatants were separated on 10% SDS–PAGE gels, electroblotted to PVDF membrane (GE Healthcare), blocked in 5% (wt/vol) milk in TBS-Tween 20 (0.1%, vol/vol), and incubated 1 h to overnight with primary antibodies (anti-GFP [1:5,000; AMS Biotechnology]). Membranes were washed 3 × 10 min in TBS-T (0.1%) before 1 h incubation in secondary antibodies (anti-rabbit HRP or AP conjugate [1:5,000; Promega]). Chemiluminescent substrate (ECL Plus, Pierce) was applied before camera detection. For AP-conjugated primary antibodies, membranes were incubated in NBT/BCIP (Roche) until bands were visible.
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