Pvp k 90

DH was provided by the Research Institute of Dong-A ST Co. Ltd. (Yongin, Korea). PVP K-90, PVP K-30, and PG were purchased from BASF (Ludwigshafen, Germany). PVA and glycerol were purchased from Sigma-Aldrich (St Louis, MO, USA). All other chemicals were of reagent-grade and used without further purification.

PVP K90 was purchased from BASF (Germany). Methotrexate (MTX, Mw = 454.44 Da) (purity >98%) was obtained from Heowns OPDE Biochemical Technology Co. Ltd. (Tianjin, China). HA was purchased from Huaxi Biotechnology Co. Ltd. (Jinan, China). Dextran (Dex) was provided by Thermo Fisher Scientific (USA). Bacillus Calmette-Guerin was purchased from the Chengdu Institute of Biological Products Co., Ltd (Sichuan,China). Trypan Blue, stearic acid, and stearyl alcohol were provided by Sinopharm Chemical Reagent Co., Ltd (China). The reagents of acetonitrile (chromatographic grade), anhydrous sodium hydrogen phosphate, liquid paraffin, Span 80, polysorbate 80, glycerinum, and sorbic acid were provided by Macklin (Shanghai, China). Citric acid was from Biotechnology Co. Ltd. (Tianjin, China).