After treatment for 2–24 h with THZ1 or DMSO, EdU was added into the cells, and the cells were incubated for 2 h. Cell proliferation was conducted using Click-iT EdU flow cytometry assay kit (Beyotime) according to the manufacturer's protocol. The stained cells were then analyzed using a flow cytometer.
Click it edu flow cytometry assay kit
Manufactured by Beyotime
The Click-iT EdU flow cytometry assay kit is a tool used for the detection and quantification of newly synthesized DNA in cells. It utilizes a chemical labeling approach to mark DNA replication, which can then be measured using flow cytometry.
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2 protocols using click it edu flow cytometry assay kit
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Cell Proliferation Assay with THZ1
2
Cell Proliferation Assay with EdU
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After the cells were treated with drugs for 24 h, EdU was added into the cells and incubated for 2 h. Cell proliferation was conducted using the Click-iT EdU flow cytometry assay kit (Beyotime) according to the manufacturer’s protocol. Then, the stained cells were analyzed by flow cytometer.
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