Five ml of sterile air was injected into the back of mice subcutaneously to generate an air pouch. Three days later another 3 ml of sterile air was injected into the pouch to maintain the air-pouch. After 3 days, 2 mg of particles in 500 μl of endotoxin-free PBS was injected into the air pouch. Control animals received 500 μl of endotoxin-free PBS. After 6 h of injection, mice were euthanized and the air pouch was lavaged with 3 ml of cold PBS. Using Shandon Cytospin centrifuge (Shandon Lipshaw) the air pouch lavage fluid cells and BALFs cells were spun down. Slides were stained with Hema-3 reagents (ThermoFisher Scientific) according to the manufacturer's recommendations.
Hema 3 reagents
Manufactured by Thermo Fisher Scientific
Hema-3 reagents are laboratory products designed for use in hematological analysis. They provide the necessary components for staining and preparation of blood samples for microscopic examination. The Hema-3 reagents facilitate the differentiation and identification of various blood cell types.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using hema 3 reagents
1
Air Pouch Inflammation Assay
2
Air Pouch Lavage Cell Staining
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