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Sc 376403

Manufactured by Santa Cruz Biotechnology
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Sc-376403 is a laboratory equipment product offered by Santa Cruz Biotechnology. It is designed for use in scientific research applications. The core function of this product is to facilitate specific tasks or processes within a laboratory setting. However, a detailed description of the product's features and capabilities cannot be provided while maintaining an unbiased and factual approach.

Automatically generated - may contain errors

Lab products found in correlation

Sc393893, by Santa Cruz Biotechnology (1 mentions) Sc 365578, by Santa Cruz Biotechnology (1 mentions) Image lab, by Bio-Rad (1 mentions) Hematoxylin, by Beyotime (1 mentions) Horseradish peroxidase hrp, by Beyotime (1 mentions) Complete protease inhibitor mixture tablet, by Roche (1 mentions) Enhanced chemiluminescence method, by Bio-Rad (1 mentions) Sc 23954, by Santa Cruz Biotechnology (1 mentions) Sonic dismembrator model 100, by Thermo Fisher Scientific (1 mentions) Sc 32233, by Santa Cruz Biotechnology (1 mentions) Pvdf membrane, by Bio-Rad (1 mentions) Exoab cd63a 1, by System Biosciences (1 mentions) M0823, by Agilent Technologies (1 mentions) Sc 166025, by Santa Cruz Biotechnology (1 mentions) Ab32391, by Abcam (1 mentions) Ab4819, by Abcam (1 mentions) Ab17942, by Abcam (1 mentions) Ab131450, by Abcam (1 mentions) Ab325572, by Abcam (1 mentions) Sc166410, by Santa Cruz Biotechnology (1 mentions)

5 protocols using sc 376403

1

Protein Expression Analysis of Angiogenic Factors

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Proteins were extracted from cells cultured in RIPA lysis buffer (RIPA, China) as described in the RT-PCR protocol. One SDS-PAGE gel was loaded with 50μg of protein per well. After the proteins were transferred on nitrocellulose membranes, antibodies specific for Dll4 (1:200, SC365429, Santa Cruz, CA), Notch1 (1:200, SC376403, Santa Cruz, CA), Notch4 (1:200, SC393893, Santa Cruz, CA) and the VEGF (1:200, SC365578, Santa Cruz, CA) were used for incubation. Band intensity was analyzed with a gel imaging analysis system (Image Lab, Bio-Rad, USA).
Gao N., Xiao L., Tao Z., Zheng Y., Wang W, & Huang H. (2022). Preliminary Research of Main Components of Dll4/ Notch-VEGF Signaling Pathway Under High-Glucose Stimulation in vitro. Diabetes, Metabolic Syndrome and Obesity: Targets and Therapy, 15, 1165-1171.
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2

Immunohistochemical Analysis of CD24 and Notch1

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Tumor samples were formalin-fixed and embedded in paraffin. Paraffin sections were stained with the first antibody for CD24 (1:200; sc-7034) and Notch1 (1:200; sc-376403) (Santa Cruz Biotechnology) by incubating overnight at 4°C. Secondary staining with biotinylated secondary antibodies with horseradish peroxidase (HRP; Beyotime Biotechnology, Nanjing, People’s Republic of China) was performed for 30 min at room temperature. Then, the sections were counterstained with hematoxylin (Beyotime).
Tang M.R., Guo J.Y., Wang D, & Xu N. (2018). Identification of CD24 as a marker for tumorigenesis of melanoma. OncoTargets and therapy, 11, 3401-3406.
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3

Extracellular Vesicle Protein Profiling

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Extracellular vesicles or C-MSCs were lyzed with radio immunoprecipitation assay (RIPA) buffer supplemented with Complete Protease Inhibitor Mixture tablets (Roche Diagnostics). The cell lysate was sonicated in ice using a sonication device (Sonic dismembrator Model100, Fisher Scientific). Five micrograms of protein of EVs or 10 μg protein of cells was separated by SDS/PAGE and transferred to PVDF membrane (BioRad). Membranes were incubated with rabbit anti-CD63 antibody (EXOAB-CD63A-1, SBI System Biosciences), mouse anti-Tsg101 antibody (sc-7964, Santa Cruz), mouse anti-calnexin antibody (sc-23954, Santa Cruz), anti-N1ICD antibody (sc-376403, Santa Cruz), or anti-GADPH antibody (sc-32233, Santa Cruz) overnight at 4°C followed by incubation with an anti-mouse or anti-rabbit goat peroxidase conjugated secondary antibody. Immunoreactive bands were visualized by the enhanced chemiluminescence method (Bio-Rad) with a western blotting detection system (Fluorchem E, ProteinSimple, United States).
Xuan W., Khan M, & Ashraf M. (2020). Extracellular Vesicles From Notch Activated Cardiac Mesenchymal Stem Cells Promote Myocyte Proliferation and Neovasculogenesis. Frontiers in Cell and Developmental Biology, 8, 11.
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4

Immunohistochemical Analysis of Vascular and Immune Markers

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Mouse monoclonal antibodies against CD31 (1:100; M0823; Dako), Notch-1 (1:50; sc-376403; Santa Cruz; CA, USA), SOX18 (1:100; sc-166025; Santa Cruz,) and CD45 (1:100; M070129-2; Dako) and rabbit polyclonal antibodies against CD68 (HPA_048982; 1:3000; Sigma) were used.
Korhonen A., Gucciardo E., Lehti K, & Loukovaara S. (2021). Proliferative diabetic retinopathy transcriptomes reveal angiogenesis, anti-angiogenic therapy escape mechanisms, fibrosis and lymphatic involvement. Scientific Reports, 11, 18810.
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5

Western Blot Analysis of Cellular Signaling

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Cells were lysed for 30 min in RIPA buffer supplemented with protease (Sigma-Aldrich, St. Louis, MO, USA) and phosphatase (PhosSTOP; Roche, Basel, Switzerland) inhibitor cocktails. Equal protein amounts of cell lysate were loaded on a 10% sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) gel, moved to polyvinylidene difluoride membranes and blocked with 5% skim milk in PBS/0.05% Tween 20 for 1 h. Primary antibodies for GAPDH (LF-PA0212, Abfrontier, Seoul, Korea), TCF4 (TCF7L2) (sc-13027, Santa Cruz Biotechnology, Dallas, TX, USA), β-catenin (ab325572, Abcam, Cambridge, MA, USA), cyclin dependent kinase 1 (CDK1) (ab131450, Abcam, Cambridge, MA, USA), cyclin D1 (sc-718, Santa Cruz Biotechnology, Dallas, TX, USA), SIRT1 (sc-15404, Santa Cruz Biotechnology), extracellular signal-regulated protein kinases 1 and 2 (ERK1/2) (ab17942, Abcam), phospho-ERK1/2 (pERK1/2) (ab4819, Abcam), glycogen synthase kinase 3 beta (GSK3β, ab32391, Abcam), caspase-3 (sc-7148, Santa Cruz Biotechnology), notch1 (sc-376403, Santa Cruz Biotechnology), and Hes1 (sc-166410, Santa Cruz Biotechnology) were used. The immunoreactive bands were viewed using horseradish peroxidase-conjugated secondary antibodies (Bio-rad) and a WEST-Queen™ RTS Western Blot Detection Kit (iNtRON Biotechnology, Seongnam, Korea) and densitometric analysis was performed.
Joo H.J., Ma D.J., Hwang J.S, & Shin Y.J. (2020). SIRT1 Activation Using CRISPR/dCas9 Promotes Regeneration of Human Corneal Endothelial Cells through Inhibiting Senescence. Antioxidants, 9(11), 1085.
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