For most biochemical experiments, mouse anti-C-CLIP-170 mAb (clone: F-3, Santa Cruz Biotechnology, Dallas, TX) was used. To detect N-terminal of CLIP-170 on western blot, custom developed rabbit anti-N-CLIP-170 polyclonal antibody (Covance, Princeton, NJ) or mouse anti-CLIP-170 (clone: E-8, Santa Cruz Biotechnology, Dallas, TX) were used. For immunofluorescence staining of endogenous CLIP-170, a mixture of mouse monoclonal antibodies 4D6 and 2D6 was used reported(Scheel et al., 1999 (link)). Other antibodies used included rabbit anti-GFP polyclonal Ab (Novus Biologicals, Littleton, CO), mouse anti-Myc mAb (clone: 9E10, Santa Cruz Biotechnology), mouse anti-31α-tubulin mAb (clone: DM1α, Sigma-Aldrich, St. Louis, MO), EB-1 Ab (clone: KT51, Abcam, Cambridge, UK) and rat anti-α-tubulin mAb (clone: YL1/2, Bio-Rad, Hercules, CA).
Docetaxel, imatinib, ponatinib, axitinib, Sunitinib and cediranib were purchased from Sigma-Aldrich (St. Louis, MO). Flutax-2 and Hexaflutax were generous gifts from Prof. Wei-Shuo Fang (Institute of Materia Medica, Beijing, China), peloruside was a gift from Dr. John Miller (Victoria University of Wellington, Wellington, New Zealand) and cyclostreptin was a gift from Dr. Chris Vanderwal (University of California, Irvine, CA).
Docetaxel, imatinib, ponatinib, axitinib, Sunitinib and cediranib were purchased from Sigma-Aldrich (St. Louis, MO). Flutax-2 and Hexaflutax were generous gifts from Prof. Wei-Shuo Fang (Institute of Materia Medica, Beijing, China), peloruside was a gift from Dr. John Miller (Victoria University of Wellington, Wellington, New Zealand) and cyclostreptin was a gift from Dr. Chris Vanderwal (University of California, Irvine, CA).