NSCLC cell line A549 (Shanghai Institute of Cell Biology, China) was cultured in Roswell Park Memorial Institute (RPMI) Medium 1640 containing 10% fetal bovine serum (FBS, Gibco Corporation, Carlsbad, CA, USA), 1 mM glutamine, 1.5 g/L sodium bicarbonate, and 1% penicillin/streptomycin. Primary antibodies included anti-B-cell lymphoma-2 (Bcl-2), anti-BCL2-Associated X (Bax), anti-poly ADP ribose polymerase (PARP)-1, anti-myeloid cell leukemia 1 (Mcl-1), anti-Bcl-xL, anti-caspase-3, anti-caspase-8, anti-caspase-9 anti-PARP, anti-AMPK, anti-p-AMPK, anti-Akt, anti-p-Akt, anti-c-Jun, Phospho-c-jun (p-c-Jun), anti-c-Jun NH2-Terminal Kinase (JNK), anti-uncoordinated 51-like kinase 1 (ULK1), anti-p-JNK, anti-mTOR, anti-Beclin-1, anti-light chain 3 (LC3), anti-sequestosome 1 (p62) and anti-GAPDH, anti-DAPI, and anti-Tom20 were purchased from Cell Signaling Technology Inc (Beverly, MA, USA). Anti-cyclin A, anti-cyclin B2, anti-cyclin D1, anti-cyclin D3, anti-p21, and anti-Cdc2 were purchased from Sigma-Aldrich (Munich, Germany), as was analytical-grade NCTD.
Anti light chain 3
Manufactured by Cell Signaling Technology
Sourced in United States
Anti-light chain 3 (LC3) is a laboratory reagent used to detect and quantify the protein LC3, which is a key marker of autophagy. LC3 plays a crucial role in the formation and maturation of autophagosomes, the double-membrane vesicles that engulf cellular components for degradation. Anti-LC3 antibodies can be used in various techniques, such as Western blotting, immunofluorescence, and immunohistochemistry, to study autophagy-related processes.
Automatically generated - may contain errors
Lab products found in correlation
Anti beclin 1, by Cell Signaling Technology (2 mentions)
Anti cyclin d1, by Merck Group (1 mentions)
Anti tom20, by Cell Signaling Technology (1 mentions)
Anti p ampk, by Cell Signaling Technology (1 mentions)
Anti akt, by Cell Signaling Technology (1 mentions)
Anti p akt, by Cell Signaling Technology (1 mentions)
Phospho cjun p cjun, by Cell Signaling Technology (1 mentions)
Anti c jun nh2 terminal kinase jnk, by Cell Signaling Technology (1 mentions)
Anti dapi, by Cell Signaling Technology (1 mentions)
Anti sequestosome 1 p62, by Cell Signaling Technology (1 mentions)
Anti p jnk, by Cell Signaling Technology (1 mentions)
Anti p21, by Merck Group (1 mentions)
Anti mtor, by Cell Signaling Technology (1 mentions)
Anti cyclin a, by Merck Group (1 mentions)
Anti cdc2, by Merck Group (1 mentions)
Anti ampk, by Cell Signaling Technology (1 mentions)
Anti bcl xl, by Cell Signaling Technology (1 mentions)
Anti gapdh, by Cell Signaling Technology (1 mentions)
Anti caspase 8, by Cell Signaling Technology (1 mentions)
Anti caspase 3, by Cell Signaling Technology (1 mentions)
2 protocols using anti light chain 3
1
NSCLC Cell Line A549 Culture Protocol
2
Protein Expression Analysis in Kidney Tissue
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The kidney tissues were homogenized in a protein lysis buffer for 20 min on ice and centrifuged at 12,000 rpm for 20 min at 4 °C. The supernatant was collected and the protein concentration was measured by the Bradford protein assay. Sodium dodecyl sulfate polyacrylamide gel electrophoresis was carried out with 8–12% polyacrylamide gels at 100 V for 1 h. The resolved proteins were transferred from the gel onto a nitrocellulose membrane (Millipore, Billerica, MA, USA) and probed with anti-TNF-α (Abcam), anti-IL-1β (Santa Cruz), anti-IL-6(Abcam), anti-fibronectin (Abcam), anti-collagen I (Abcam), anti- α-SMA (Abcam), anti-Beclin-1 (Cell Signaling Technology, Beverly, MA, USA), anti-p62 (Cell Signaling Technology), anti-light chain 3 (LC3) (Cell Signaling Technology), anti-cleaved-caspase3 (Cell Signaling Technology), anti-cleaved-poly (ADP-ribose) polymerase1 (PARP1, Santa Cruz), anti-p53 (Santa Cruz), and anti-glyceraldehyde 3-phosphate dehydrogenase (GAPDH, Cell Signaling Technology). This was followed by a secondary antibody conjugated to horseradish peroxidase (1:1000) and determined with enhanced chemiluminescence reagents (Amersham Biosciences, Piscataway, NJ, USA). The signal intensity was quantified by an image analyzer (Chemidoc XRS+ system; Bio-Rad Laboratories, Hercules, CA, USA).
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