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Agilent haloplex custom target enrichment

Manufactured by Agilent Technologies
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The Agilent Haloplex Custom Target Enrichment is a targeted enrichment solution for next-generation sequencing. It allows for the selective capture and sequencing of specific regions of the genome.

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Lab products found in correlation

Hiseq 2500, by Illumina (2 mentions) Taqman snp assay, by Thermo Fisher Scientific (2 mentions)

Spelling variants of this product

HaloPlex (35 citations)

2 protocols using agilent haloplex custom target enrichment

1

Targeted Sequencing for Rare Variants in AVSD

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Genes enriched for rare (including novel) and rare/damaging non-synonymous variants in AVSD cases vs. EVS controls on mutation burden analysis were selected for targeted sequencing in the replication cohort using Agilent Haloplex Custom Target Enrichment (Agilent Technologies Inc., Santa Clara, CA). A custom probe set was designed using Agilent SureDesign web-based software to target all coding exons for the prioritized genes (earray.chem.agilent.com/suredesign). Sequencing (100 bp paired-end reads, targeted at >200X coverage) was performed using Illumina HiSeq2500 (Illumina Inc., San Diego, CA) at The Centre for Applied Genomics (TCAG) in The Hospital for Sick Children. Agilent SureCall software was used to generate BAM files and perform variant calling. Variants were annotated and filtered using the same protocol as for the primary cohort. To assess the population frequency of the recurring variant in MDM4 [8:204518457 A>C (K324Q, rs41299595)], genotyping was performed in 97 Caucasian controls without heart disease from Ontario using a custom-designed TaqMan® SNP Assays (Life Technologies Corporation) (Table S2.2). Samples were analyzed using the ViiA™ 7 Real-Time PCR System with ViiA™7 software.
D’Alessandro L.C., Al Turki S., Manickaraj A.K., Manase D., Mulder B.J., Bergin L., Rosenberg H.C., Mondal T., Gordon E., Lougheed J., Smythe J., Devriendt K., Bhattacharya S., Watkins H., Bentham J., Bowdin S., Hurles M.E, & Mital S. (2015). Exome Sequencing Identifies Rare Variants in Multiple Genes in Atrioventricular Septal Defect. Genetics in medicine : official journal of the American College of Medical Genetics, 18(2), 189-198.
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2

Targeted Sequencing for Rare Variants in AVSD

Check if the same lab product or an alternative is used in the 5 most similar protocols
Genes enriched for rare (including novel) and rare/damaging non-synonymous variants in AVSD cases vs. EVS controls on mutation burden analysis were selected for targeted sequencing in the replication cohort using Agilent Haloplex Custom Target Enrichment (Agilent Technologies Inc., Santa Clara, CA). A custom probe set was designed using Agilent SureDesign web-based software to target all coding exons for the prioritized genes (earray.chem.agilent.com/suredesign). Sequencing (100 bp paired-end reads, targeted at >200X coverage) was performed using Illumina HiSeq2500 (Illumina Inc., San Diego, CA) at The Centre for Applied Genomics (TCAG) in The Hospital for Sick Children. Agilent SureCall software was used to generate BAM files and perform variant calling. Variants were annotated and filtered using the same protocol as for the primary cohort. To assess the population frequency of the recurring variant in MDM4 [8:204518457 A>C (K324Q, rs41299595)], genotyping was performed in 97 Caucasian controls without heart disease from Ontario using a custom-designed TaqMan® SNP Assays (Life Technologies Corporation) (Table S2.2). Samples were analyzed using the ViiA™ 7 Real-Time PCR System with ViiA™7 software.
D’Alessandro L.C., Al Turki S., Manickaraj A.K., Manase D., Mulder B.J., Bergin L., Rosenberg H.C., Mondal T., Gordon E., Lougheed J., Smythe J., Devriendt K., Bhattacharya S., Watkins H., Bentham J., Bowdin S., Hurles M.E, & Mital S. (2015). Exome Sequencing Identifies Rare Variants in Multiple Genes in Atrioventricular Septal Defect. Genetics in medicine : official journal of the American College of Medical Genetics, 18(2), 189-198.
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