The cell lysate containing the recombinant rfsp was separated by 12% SDS-PAGE and electro-transferred onto PVDF membrane (Roche). The membrane was blocked using 5% non-fat skim milk in TBS buffer (50 mM Tris-Cl, 150 mM NaCl, pH: 7.5) with 0.05% Tween 20 (37°C, 2 h). Then, incubation was done with HRP-conjugated mouse anti-poly His-tag antibody (1:2000 Roche). was used to incubate the membrane. For signal development, Stain the membrane with 0.05% 3, 3’-Diaminobenzidine tablet (DAB Reagents; Sigma) 0.015% H2O2 in 0.01 M PBS, PH 7. 2. Bands will appear after 2 minutes.
Hrp conjugated mouse anti poly his tag antibody
Manufactured by Roche
The HRP-conjugated mouse anti-poly His-tag antibody is a laboratory reagent designed for the detection of proteins tagged with a histidine (His) sequence. It consists of a mouse-derived antibody conjugated with horseradish peroxidase (HRP), which enables colorimetric or chemiluminescent detection of the target His-tagged proteins.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using hrp conjugated mouse anti poly his tag antibody
1
Recombinant Protein Detection by Western Blot
2
Protein Separation and Detection using SDS-PAGE and Western Blot
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
To separate the RS protein, SDS-PAGE with a concentration of 12% was employed. The protein bands were then electro-transferred from the gel onto a PVDF membrane (Roche). Following the transfer, the PVDF membrane was subjected to blocking using 5% non-fat skim milk in TBS buffer (50 mM Tris-Cl, 150 mM NaCl, pH 7.5) containing 0.05% Tween 20. The blocking step was carried out at 37°C for 2 hours. For protein detection, the PVDF membrane was incubated with HRP-conjugated mouse anti-poly His-tag antibody (1:2000, Roche). Subsequently, the membrane was immersed in a solution containing 3,3'-Diaminobenzidine (DAB Reagents, Sigma) to develop the signal and visualize the protein bands on the membrane.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!