Ammonium bicarbonate, DTT, iodoacetamide, sodium chloride, formic acid, acetonitrile, trifluoroacetic acid, acetic acid, and thiourea were from Sigma-Aldrich (Milan, Italy), while Tris and urea from Euroclone. Trypsin was purchased from Promega (Sequencing Grade Modified Trypsin) and Lys-C from Thermo Scientific (MS grade). The hand-made desalting/purification STAGE column were prepared using three C18 Empore Extraction Disks (3M).
Sequencing grade modified trypsin
Manufactured by Thermo Fisher Scientific
Sequencing Grade Modified Trypsin is a proteolytic enzyme used in protein digestion and sample preparation for mass spectrometry and protein sequencing analysis. It is optimized for efficient and reproducible protein cleavage.
Automatically generated - may contain errors
Lab products found in correlation
Lys c, by Thermo Fisher Scientific (2 mentions)
Tris and urea, by Euroclone (1 mentions)
Dithiothreitol (dtt), by Merck Group (1 mentions)
Formic acid, by Merck Group (1 mentions)
Iodoacetamide, by Merck Group (1 mentions)
Acetonitrile, by Merck Group (1 mentions)
Acetic acid, by Merck Group (1 mentions)
Sodium chloride (nacl), by Merck Group (1 mentions)
Trifluoroacetic acid, by Merck Group (1 mentions)
Ammonium bicarbonate, by Merck Group (1 mentions)
Thiourea, by Merck Group (1 mentions)
Nitrocellulose membrane, by Euroclone (1 mentions)
Tween20, by Euroclone (1 mentions)
Urea, by Euroclone (1 mentions)
Empore c18 extraction disks, by 3M (1 mentions)
Trypsin, by Promega (1 mentions)
2 protocols using sequencing grade modified trypsin
1
Protein Sample Preparation Protocol
2
Reagents and Materials for Proteomic Analysis
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
All reagents were purchased from Sigma-Aldrich (Milano, Italy) and were of reagent grade.
Tris, glycine, Tween-20, urea, nitrocellulose membrane were from Euroclone. Trypsin was from Promega (Sequencing Grade Modified Trypsin) and Lys-C from Thermo Scientific (MS grade). The hand-made desalting/purification STAGE (STop And Go Extraction) tips were prepared using three C18 Empore Extraction Disks (3M) 37, 38 .
Tris, glycine, Tween-20, urea, nitrocellulose membrane were from Euroclone. Trypsin was from Promega (Sequencing Grade Modified Trypsin) and Lys-C from Thermo Scientific (MS grade). The hand-made desalting/purification STAGE (STop And Go Extraction) tips were prepared using three C18 Empore Extraction Disks (3M) 37, 38 .
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!