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Rabbit anti histone3

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Rabbit anti-Histone3 is a primary antibody that recognizes histone H3, a core component of the nucleosome in eukaryotic cells. This antibody can be used in various applications, such as Western blotting, immunohistochemistry, and immunocytochemistry, to detect and study histone H3 expression and localization.

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Lab products found in correlation

Goat anti rabbit igg hrp conjugate, by Thermo Fisher Scientific (2 mentions) Goat anti mouse igg hrp conjugate, by Thermo Fisher Scientific (2 mentions) Mouse anti α tubulin, by Merck Group (2 mentions) Rabbit anti gapdh, by Proteintech (2 mentions) Ecl western blotting kit, by GE Healthcare (1 mentions) Sds loading buffer, by Bio-Rad (1 mentions) Mouse anti ty1, by Merck Group (1 mentions) Rabbit anti pfaldolase, by Abcam (1 mentions) Mouse anti ha, by Roche (1 mentions)

3 protocols using rabbit anti histone3

1

Western Blot Analysis of Small Animals

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Individual 1–3mm sized animals were homogenized in protein loading buffer (60mM Tris-Cl pH6.8, 5% Glycerol, 1% SDS and 2.5% β-mercaptoethanol) and separated on 8% denaturing polyacrylamide gel. Samples were transferred to PVDF membrane, blocked, and incubated with the primary antibody in PBSTw containing 1.5% milk. The following commercial antibodies were used: mouse anti-α-tubulin (Millipore) at 1:10000; rabbit anti-GAPDH (Proteintech) at 1:3000, rabbit anti-Histone3 (Abcam) at 1:2000. Secondary antibodies goat anti-Rabbit IgG HRP Conjugate or goat anti-Mouse IgG HRP Conjugate (Life Technologies) were used at 1:10000 in PBSTw containing 1.5% milk.
Li D., Taylor D.H, & van Wolfswinkel J.C. (2021). PIWI-mediated control of tissue-specific transposons is essential for somatic cell differentiation. Cell reports, 37(1), 109776.
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2

Western Blot Analysis of Parasite Proteins

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Total parasite extracts were prepared by 0.15% saponin treatment and resuspended in 1x SDS-loading buffer (Bio-Rad), then separated on 8 ~ 12% SDS-PAGE gel according to the molecular weight of targeted proteins, and subjected to Western blot analysis. The commercial antibodies used in this study were mouse anti-Ty1 (1:500, Sigma), mouse anti-HA (1:2000, Roche), rabbit anti-PfAldolase (1:1000, Abcam), and rabbit anti-Histone 3 (1:1000, Abcam). ECL western blotting kit (GE healthcare) was used to develop blots.
Droll D., Wei G., Guo G., Fan Y., Baumgarten S., Zhou Y., Xiao Y., Scherf A, & Zhang Q. (2018). Disruption of the RNA exosome reveals the hidden face of the malaria parasite transcriptome. RNA Biology, 15(9), 1206-1214.
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3

Western Blot Analysis of Small Animals

Check if the same lab product or an alternative is used in the 5 most similar protocols
Individual 1–3mm sized animals were homogenized in protein loading buffer (60mM Tris-Cl pH6.8, 5% Glycerol, 1% SDS and 2.5% β-mercaptoethanol) and separated on 8% denaturing polyacrylamide gel. Samples were transferred to PVDF membrane, blocked, and incubated with the primary antibody in PBSTw containing 1.5% milk. The following commercial antibodies were used: mouse anti-α-tubulin (Millipore) at 1:10000; rabbit anti-GAPDH (Proteintech) at 1:3000, rabbit anti-Histone3 (Abcam) at 1:2000. Secondary antibodies goat anti-Rabbit IgG HRP Conjugate or goat anti-Mouse IgG HRP Conjugate (Life Technologies) were used at 1:10000 in PBSTw containing 1.5% milk.
Li D., Taylor D.H, & van Wolfswinkel J.C. (2021). PIWI-mediated control of tissue-specific transposons is essential for somatic cell differentiation. Cell reports, 37(1), 109776.
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