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Uv 2401pc ultraviolet spectrophotometer

Manufactured by Shimadzu
Sourced in Japan

The UV-2401PC is an ultraviolet spectrophotometer manufactured by Shimadzu. The device is designed to measure the absorbance or transmittance of samples in the ultraviolet and visible light spectrum.

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3 protocols using uv 2401pc ultraviolet spectrophotometer

1

Oxidative Stress Biomarkers in Liver and Serum

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The levels of total superoxide dismutase (T-SOD), catalase (CAT), glutathione (GSH), glutathione peroxidase (GSH-PX), malonaldehyde (MDA) and total protein (TP) in liver or serum were determined. The absorbance was measured by UV-2401PC ultraviolet spectrophotometer (Shimadzu Corporation, Japan). The determination were operated according to the guidelines of commercial kits.
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2

Biochemical Serum Marker Quantification

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We tested the serum levels of albumin (ALB), low density lipoprotein cholesterol (LDL), blood urea nitrogen (BUN) and alkaline phosphatase (AKP) by using the commercial kit (Nanjing Jiancheng Bioengineering Institute, Nanjing, China). The absorbance was measured by UV-2401PC ultraviolet spectrophotometer (Shimadzu Corporation, Japan). The sample processing, determination and calculation of the results were carried out in accordance with the operating steps of the kit instructions.
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3

Cytokine and Immunoglobulin Profiles in Liver

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Interleukin-1β (IL-1β), Interleukin-6 (IL-6), Interleukin-8 (IL-8), Interleukin-12 (IL-12) and Tumor necrosis factor-α (TNF-α) were detected in the liver. In addition, the immunoglobulin A (IgA), immunoglobulin M (IgM) and immunoglobulin G (IgG) in serum were detected. The absorbance was measured by UV-2401PC ultraviolet spectrophotometer (Shimadzu Corporation, Japan).
The assays were performed according to the instructions of commercial kits.
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