The following antibodies were raised in sheep and affinity-purified on the appropriate antigen by the Division of Signal Transduction Therapy Unit at the University of Dundee: KCC2A phospho-Thr 906 (SAYTYER(T)LMMEQRSRR [residues 975 – 989 of human KCC3A] corresponding to SAYTYEK(T)LVMEQRSQI [residues 899 – 915 of human KCC2A]) (Catalog number: S959C); KCC2A phospho-Thr 1007 (CYQEKVHM(T)WTKDKYM [residues 1032 – 1046 of human KCC3A] corresponding to TDPEKVHL(T)WTKDKSVA [residues 998 – 1014 of human KCC2A]) (Catalog number: S961C); KCC2 phospho-Ser940 (Catalog number: NBP2–29513). Pan KCC2 total antibody (residues 932–1043 of human KCC2) was purchased from NeuroMab (Catalog number: 73–013). Anti-β-Tubulin III (neuronal) antibody was purchased from Sigma-Aldrich (Catalog number: T8578). Secondary antibodies coupled to horseradish peroxidase used for immunoblotting were obtained from Pierce. IgG used in control immunoprecipitation experiments was affinity-purified from pre-immune serum using Protein G-Sepharose.
Anti β tubulin 3 neuronal antibody
Manufactured by Merck Group
Sourced in United States
The Anti-β-Tubulin III (neuronal) antibody is a laboratory tool designed to detect the presence of the β-Tubulin III protein, which is specifically expressed in neuronal cells. This antibody can be used as a marker to identify and study neuronal cells in various biological samples.
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Lab products found in correlation
2 protocols using anti β tubulin 3 neuronal antibody
1
Affinity-purified Antibodies for KCC2 Phosphorylation
2
Immunostaining of Cultured Neurons
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The culture medium was discarded, and the neurons were washed 3 times with PBS. The cultured neurons were perfused with 4% buffered paraformaldehyde (PFA) for 30 min, washed 3 times with PBS, and then incubated in blocking solution (0.1% Triton X-100 + 10% goat block serum + PBS) for 1 h. Following this step, the cultured neurons were incubated with the primary antibodies anti-β-Tubulin III (neuronal) antibody (Cat no. T8578; 1:5000; Sigma, Saint Louis, USA) or anti-BACE1 antibody (catalog #ab2077; 1:1000; Abcam) overnight at 4°C. The neurons were then washed and incubated with the secondary antibodies conjugated to Alexa Fluor 488 and Alexa Fluor 594 (Molecular Probes, Eugene, OR, USA) for 1 h at room temperature. The immunofluorescence intensity of BACE1 was measured by image Plus 5.0 software.
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