Anti cxcr4

Manufactured by LifeSpan BioSciences

Sourced in United States

Anti-CXCR4 is a laboratory reagent used in research applications to detect and quantify the CXCR4 protein. CXCR4 is a chemokine receptor that plays a role in various biological processes. This reagent can be utilized in techniques such as flow cytometry, immunohistochemistry, and ELISA to study the expression and distribution of CXCR4 in different cell types and samples.

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Lab products found in correlation

Bca protein assay kit, by Thermo Fisher Scientific (2 mentions) Anti ccr1, by R&D Systems (1 mentions) Anti ccr8, by R&D Systems (1 mentions) Anti flag, by Merck Group (1 mentions) Enhanced chemiluminescent reagent, by Thermo Fisher Scientific (1 mentions) Anti colii, by Abcam (1 mentions) Horseradish peroxidase conjugated goat anti rabbit immunoglobulin g, by Abcam (1 mentions) Anti β actin, by Abcam (1 mentions)

2 protocols using anti cxcr4

Chemokine Receptor Interaction Assay

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Chemokine (C-C motif) ligand (CCL) 1 (CCL1), CCL2 and chemokine (C-X-C motif) ligand 12 (CXCL12) were purchased from Protein Foundry (Milwaukee, WI). Antibodies were obtained from Abcam (Cambridge, United Kingdom): anti-α_1B-AR (host: rabbit; catalog: ab169523), anti-α_1D-AR (host: rabbit; catalog: ab84402), anti-CXCR4 (host: goat, ab1670), anti-HA (host: rabbit; catalog: ab9110) ; LifeSpan Biosciences (Seattle, WA): anti-CCR8 (host: goat; catalog: LS-C187704); R&D Systems (Minneapolis, MN): anti-CCR1 (host: mouse; catalog: MAB145), anti-CCR2 (host: mouse; catalog: MAB48607), allophycocyanin (APC) conjugated anti-mouse CD45 (host: rat; catalog: FAB114A), APC conjugated Immunoglobulin G 2B (IgG2B) isotype control (host: rat; catalog: ICO13A), Immunoglobulin G (IgG) isotype control (host: rabbit; catalog: MAB150), IgG isotype control (host: mouse; catalog: MAB004), and IgG isotype control (host: goat; catalog: AB-108-C); and Sigma-Aldrich (St. Louis, MO): anti- FLAG (host: mouse; catalog: F3165).
Phenylephrine (PE), phentolamine (PT), norepinephrine (NE), lipopolysaccharide (LPS) from Pseudomonas aeruginosa (serotype 10.22; source strain ATCC 27316) and all proximity ligation assay (PLA) reagents were purchased from Sigma-Aldrich.

Gao X., Enten G.A., McGee M.Y., Weche M, & Majetschak M. (2023). α1-adrenoceptor ligands inhibit chemokine receptor heteromerization partners of α1B/D-adrenoceptors via interference with heteromer formation. Pharmacological research, 190, 106730.

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Western Blot Analysis of Protein Expression

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Total protein was extracted from cells following lysis with radioimmunoprecipitation assay buffer and quantified by the BCA Protein Assay kit (Thermo Fisher Scientific, Inc.). Total protein (30 µg) was separated via electrophoresis on 10% SDS-PAGE gels prior to transfer to polyvinylidene fluoride membranes. Membranes were probed with primary antibodies in TBS with 5% non-fat milk. The antibodies included were anti-CXCR4 (LifeSpan BioSciences, Inc., Seattle, WA, USA; 1:1,000 dilution; cat. no. LS-B2160-0.05), anti-Col II (Abcam, Cambridge, UK; 1:1,000 dilution; cat. no. ab188570) and anti-β-actin (Abcam, Cambridge, UK; 1:5,000 dilution; cat. no. ab8227), and horseradish peroxidase-conjugated goat anti-rabbit immunoglobulin G (Abcam, Cambridge, UK; 1:10,000 dilution; cat. no. ab97051) was used as the secondary antibody. Proteins of interest were visualized using enhanced chemiluminescent reagent (Thermo Fisher Scientific, Inc.). The band intensities were quantified by densitometry using ImageJ 1.46r software (National Institutes of Health, Bethesda, MD, USA) (41 (link)). Experiments were repeated at least 3 times.

Jia D., Li Y., Han R., Wang K., Cai G., He C, & Yang L. (2019). miR-146a-5p expression is upregulated by the CXCR4 antagonist TN14003 and attenuates SDF-1-induced cartilage degradation. Molecular Medicine Reports, 19(5), 4388-4400.

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