The cytokines in the culture supernatants were detected with Mouse IL-17 DuoSet ELISA Kit (R&D Systems) according to the manufacturer’s instructions. Briefly, 96-well plates were coated with the capture antibodies overnight at room temperature. After washing and blocking, the diluted supernatants and recombinant cytokine standards were added to the plates and incubated for 2 h at room temperature. Then, the plates were incubated sequentially with the detection antibodies and streptavidin-HRP, as well as Substrate Reagent (R&D Systems) and Stop Solution. The absorbance was measured at 450 nm with wavelength correction set to 540 nm.
Mouse il 17 duoset elisa kit
Manufactured by R&D Systems
Sourced in United States
The Mouse IL-17 DuoSet ELISA Kit is a sandwich enzyme-linked immunosorbent assay (ELISA) for the quantitative measurement of mouse Interleukin-17 (IL-17) in cell culture supernatants, serum, and plasma.
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3 protocols using mouse il 17 duoset elisa kit
1
Cytokine Detection in Cell Culture Supernatants
2
Cytokine Detection in Cell Supernatants
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The cytokines in the culture supernatants were detected with Mouse IL-17 DuoSet ELISA Kit (R&D Systems, Minneapolis, MN, USA) and Mouse IFN-gamma DuoSet ELISA Kit (R&D Systems) according to the manufacturer’s instructions. Briefly, 96-well plates were coated with the capture antibodies overnight at room temperature. After washing and blocking, the diluted supernatants and recombinant cytokine standards were added to the plates and incubated for 2 h at room temperature. Then, the plates were incubated sequentially with the detection antibodies and streptavidin-HRP, as well as Substrate Reagent (R&D Systems) and Stop Solution. The absorbance was measured at 450 nm with wavelength correction set to 540 nm.
3
Cytokine Profiling in Mice
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IL-17 serum concentrations were determined using the mouse IL-17 DuoSet ELISA kit (R&D Systems); mouse IL-10 concentrations using the Quantikine ELISA kit (R&D Systems). IFNγ, TNFα, IL-6, IL-12p70, IL-4 and IL-5 serum levels were quantified using the ProcartaPlex™ Mouse Th1/Th2 essential 6plex Immunoassay (eBioscience).
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