Alamethicin

Manufactured by Meilun

Sourced in China

Alamethicin is a naturally occurring peptide that exhibits ion channel-forming properties. It is isolated from the fungus Trichoderma viride. Alamethicin has the ability to form voltage-dependent, ion-permeable channels in lipid bilayer membranes.

Automatically generated - may contain errors

Lab products found in correlation

Nicotinamide adenine dinucleotide phosphate nadph, by Yuanye Bio-Technology (1 mentions) Dimethyl sulfoxide (dmso), by Meilun (1 mentions) Ammonium acetate, by Sinopharm (1 mentions) Tectorigenin, by Yuanye Bio-Technology (1 mentions) Trifluoperazine, by Merck Group (1 mentions) Irigenin, by Yuanye Bio-Technology (1 mentions) Fluconazole, by Merck Group (1 mentions) 1 naphtol, by Merck Group (1 mentions) Udpga, by Merck Group (1 mentions) β glycosidase, by Merck Group (1 mentions) Silibinin, by Merck Group (1 mentions)

2 protocols using alamethicin

In-House Synthesis and Characterization of ARV-471

Check if the same lab product or an alternative is used in the 5 most similar protocols

ARV-471 was synthesized in-house, and its identity and purity were assessed using nuclear magnetic resonance spectroscopy, mass spectrometry, and high-performance liquid chromatography. Dog liver microsome (DLM) was purchased from Corning Gentest (Glendale, AZ, USA). 7-Ethoxycoumarin (7-EC) was purchased from J&K Scientific Ltd. (Shanghai, China). Phosphate buffer saline (PBS), MgCl 2 , DMSO, alamethicin, and uridine-5'-diphosphoglucuronic acid (UDPGA) were from Meilunbio (Dalian, China). Nicotinamide adenine dinucleotide phosphate (NADPH) was purchased from Shanghai Yuanye Bio-Technology Co., Ltd.
(Shanghai, China). Ammonium acetate was from Sinopharm Group Co., Ltd.
(Shanghai, China). Formic acid was purchased from RHAWN (Shanghai, China).
Acetonitrile (ACN) of LC-MS grade was obtained from Sigma-Aldrich (St. Louis, MO, USA). Ultrapure water was freshly prepared with a Millipore purification system (Molsheim, France).

He Y., Hou P., Long Z., Zheng Y., Tang C., Jones E., Diao X, & Zhu M. (2024). Application of Electro-Activated Dissociation Fragmentation Technique to Identifying Glucuronidation and Oxidative Metabolism Sites of Vepdegestrant by Liquid Chromatography-High Resolution Mass Spectrometry. Drug metabolism and disposition: the biological fate of chemicals, 52(7).

+ Open protocol

+ Expand

In Vitro Glucuronidation Assay

Check if the same lab product or an alternative is used in the 5 most similar protocols

Tectorigenin and irigenin were supplied by Yuanye Biotechnology Co., Ltd. (Shanghai, China). UDPGA, silibinin, 1-naphtol, fluconazole, trifluoperazine, and β-glycosidase were obtained from Sigma-Aldrich (St. Louis, MO, USA). Alamethicin and d-glucaric acid 1,4-lactone monohydrate were purchased from Meilunbio (Shanghai, China). Pooled HLMs (mixed gender, protein concentration: 15 mg/mL) were purchased from the Research Institute for Liver Diseases (Shanghai, China). Nine recombinant human UGT enzymes (UGT1A1/1A3/1A4/1A6/1A9/2B4/2B7/2B15/2B17) were obtained from BD Biosciences (Woburn, MA, USA). HLMs and recombinant UGTs were stored in a freezer (−80 °C) until use. All other reagents were supplied by the Sinopharm Group (Shanghai, China).

Li J., Xu Z, & Gu J. (2022). UGT1A1 and UGT1A9 Are Responsible for Phase II Metabolism of Tectorigenin and Irigenin In Vitro. Molecules, 27(13), 4104.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!