The serum exosomes16 (link)–18 (link) were isolated using supercentrifugation (L-90KL-80XP, Beckman Coulter, California, America). Briefly, the serum samples were first centrifuged at 2000 g for 30 min to remove died cells and debris, then centrifuged at 100,000 g for 70 min, supernatant was discarded. The sediment was washed by 1 ml phosphate-buffered saline (PBS) and were centrifuged at 100,000 g for 70 min19 (link), supernatant was discarded and the remaining was mainly serum exosomes. Finally, serum exosomes were resuspended with 200μL PBS for identification and RNA extraction.
L 90kl 80xp
Manufactured by Beckman Coulter
The L-90KL-80XP is a high-performance ultracentrifuge designed for a variety of laboratory applications. It features a maximum speed of 90,000 RPM and a maximum RCF of 800,000 x g, making it capable of separating particles and macromolecules with exceptional precision and resolution.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using l 90kl 80xp
1
Serum Exosome Isolation and Characterization
2
Exosome Isolation via Ultracentrifugation
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The exosomes were isolated using supercentrifugation (L-90KL-80XP, Beckman Coulter, America). Briefly, the serum samples were first centrifuged at 2000g for 30 min to remove died cells and debris, then centrifuged at 100000 g for 70 min. After that, supernatant was discarded. 1 ml phosphate-buffered saline (PBS) was added to wash the sediment. Sediment were centrifuged at 100000 g for 70 min again, supernatant was discarded. Finally, exosomes were resuspended with 200μL PBS for identification and RNA extraction.
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