Sh igf2bp1

short hairpin RNA (Sh)-METTL14, sh-YTHDF1, sh-YTHDF2, sh-YTHDC1, sh-IGF2BP1, sh-IGF2BP2, sh-IGF2BP3, sh-NC, empty vector, and SMAD1 overexpression vector, IGF2BP1 overexpression vector, and IGF2BP2 overexpression vector were obtained from Genepharma. BMSCs were seeded into 6-well plates and transfected with sh-METTL14 and sh-NC using Lipofectamine 3000 (Invitrogen, Carlsbad) for 48 h.

In this study, lipopolysaccharide (LPS, #L2880) and MIF antagonist (ISO-1, #475837) were bought from Sigma-Aldrich (Missouri, USA). Cycloheximide (CHX, #HY-12320) and 3-Deazaadenosine (DAA, #HY-W013332) were purchased from MedChemExpress (MCE, New Jersey, USA). VX765 (#inh-vx765i) was purchased from Invivogen (California, USA). The sh-IGF2BP1, sh-METTL3, sh-MIF, sh-E2F1, and sh-NC were obtained from Genepharma company (Shanghai, China). Lentiviruses expressing sh-IGF2BP1 and nonsense control (NC) were purchased from Tsingke (Nanjing, China). The E2F1 overexpression vector was constructed using p-ENTER, while the overexpression plasmids for MIF or METTL3 were constructed in pcDNA3.1.