The porous PDMS layer with haircell structures was fabricated by a well-known polymer molding technique. In order to facile demold the porous PDMS layer with haircell structures from the mold, trichloro(1H,1H,2H,2H-perfluorooctyl)silane (Sigma Aldrich) was vaporized on the mold under vacuum condition for 50 min. Subsequently, the PDMS with crystallized citric acid powders and a curing agent were mixed in a weight ratio of 15 : 1 and spin-coated on the silane treated mold for the thickness of 40 μm, followed by solidification at 100 °C for 1 h. The citric acid powder in PDMS was dissolved in ethanol for 1 min 30 s to form the porous PDMS layer with haircell structures on the mold.
Trichloro 1h
Manufactured by Merck Group
Sourced in Germany, United States
Trichloro(1H-pyrrol-2-yl)silane is a laboratory chemical compound primarily used as a reagent in organic synthesis. It serves as a precursor for the preparation of various pyrrol-containing molecules. The core function of this product is to provide a silylating agent for the introduction of silyl groups onto organic compounds.
Automatically generated - may contain errors
Lab products found in correlation
H perfluorooctyl silane, by Merck Group (1 mentions)
4 6 diamidino 2 phenylindole dihydrochloride dapi, by Merck Group (1 mentions)
Phosphate buffered saline (pbs), by Merck Group (1 mentions)
Polydiallyldimethylammonium chloride, by Merck Group (1 mentions)
Trichloro 1, by Merck Group (1 mentions)
Hfe 7500, by Merck Group (1 mentions)
4 protocols using trichloro 1h
1
Fabrication of Porous PDMS Haircells
2
Microfluidic Chip Fabrication for Cell Clustering
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The microfluidic chips were fabricated using standard soft-lithography procedures in Polydimethylsiloxane (PDMS) (SYLGARD™ 184 silicone elastomer kit) [[65] (link), [67] (link)]. PDMS molds were made via double-casting by thoroughly mixing the base resin and curing agent in a ratio of 10:1 (w/w). After plasma treatment, the replica PDMS mold was exposed to trichloro (1H,1H,2H,2H-perfluorooctyl) silane (Sigma-Aldrich, # 448931, Germany) within a vacuum desiccator for at least 6 h. Then the layers were baked for 2 h at 70 °C to get a final device [17] . The final microwell was 500 μm (I.D) × 500 μm (depth) × 2,000 μm (thickness) (Supplementary Figure 1-2). Cells can form compact clusters in our tapered microwell assay (Supplementary Figure 3). Besides, the inside walls of our microwell could provide sufficient depth to avoid turbulence incurred from media change while concentrating the cells for cluster formation [16] (link), [18] (link), [19] , [20] , [66] .
3
Fluorinated Surfactants and Biomolecule Protocols
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Native BSA, aBSA, and cBSA
were prepared and provided, as described in the literature.21 (link) The fluorinated surfactant 2,3,4,5,6-perfluorobenzoyl
chloride, PBS, trichloro (1H,1H,2H,2H-perfluorooctyl) silane (97%), the
1H,1H,2H,2H-perfluorodecanethiol (97%), and the nuclear staining agent
4′,6-diamidino-2-phenylindole dihydrochloride (DAPI) were purchased
from Sigma-Aldrich Co. The fluorinated oil (Novec 7500) was obtained
from ACOTA. The SPR-Au chips were obtained from Ssens.
were prepared and provided, as described in the literature.21 (link) The fluorinated surfactant 2,3,4,5,6-perfluorobenzoyl
chloride, PBS, trichloro (1H,1H,2H,2H-perfluorooctyl) silane (97%), the
1H,1H,2H,2H-perfluorodecanethiol (97%), and the nuclear staining agent
4′,6-diamidino-2-phenylindole dihydrochloride (DAPI) were purchased
from Sigma-Aldrich Co. The fluorinated oil (Novec 7500) was obtained
from ACOTA. The SPR-Au chips were obtained from Ssens.
4
Microfluidic Device Fabrication and Surface Modification
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We produce flow focussing microfluidic devices from poly(dimethylsiloxane) (PDMS) (Dow Corning, USA) using soft lithography. 1 The channel walls of single emulsion drop makers are rendered fluorophilic by injecting a HFE-7500-based solution containing 2 vol% of trichloro(1H,1H,2H,2H-perfluorooctyl)silane (Sigma-Aldrich, USA) for 10 min. To modify the surface of double emulsion drop makers, their channel walls are activated with 1M NaOH for 10 min before they are dried with compressed air. The first junction is rendered fluorophilic by treating this part of the channel with a HFE-7500-based solution containing 2 vol% of trichloro(1H,1H,2H,2H-perfluorooctyl)silane (Sigma-Aldrich, USA). The second junction, which is a 3D junction, 2 is rendered hydrophilic using an aqueous solution containing 2 wt.% polydiallyldimethylammonium chloride (Sigma-Aldrich, USA). The solutions are left in the channels for 30 min before channels are dried with compressed air.
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