Total RNA was isolated by use of RNeasy Plus Mini Kit (Qiagen) according to the manufacturer′s instructions. Reverse transcription was performed using the QuantiTec Reverse Transcription Kit (Qiagen) following the instructions for 1 μg RNA. Quantitative PCR was performed as described in Eberle et al. (48 (link)) using SensiFAST Probe LO-ROX mix (2×) from the SensiFAST Probe LO-ROX Kit (Bioline, London, UK). The PCR was performed on the qTower (Analytik Jena, Jena, Germany) by using the program 2 min 95°C and 40 cycles of 10 s 95°C and 20 s of 60°C. Controls were PPIA HEX, RPLP0 HEX, YWHAZ HEX (all three BioRad Laboratories, San Diego, USA). The genes of interest were analyzed with REV3L Fam, REV1 Fam, SMARCAL1 Fam, ZRANB3 Fam (BioRad Laboratories, San Diego, USA).
Rev3l fam
Manufactured by Bio-Rad
Sourced in United States
The REV3L Fam is a laboratory equipment product from Bio-Rad. It is a thermal cycler designed for nucleic acid amplification, specifically real-time PCR (polymerase chain reaction) experiments.
Automatically generated - may contain errors
Lab products found in correlation
Qtower, by Analytik Jena (2 mentions)
Ppia hex, by Bio-Rad (2 mentions)
Quantitec reverse transcription kit, by Qiagen (2 mentions)
Rneasy plus mini kit, by Qiagen (2 mentions)
Sensifast probe lo rox mix, by Meridian Bioscience (1 mentions)
Sensifast probe lo rox kit, by Meridian Bioscience (1 mentions)
Ywhaz hex, by Bio-Rad (1 mentions)
Gapdh hex, by Bio-Rad (1 mentions)
2 protocols using rev3l fam
1
Quantitative PCR Gene Expression Analysis
2
Quantitative PCR Analysis of Gene Expression
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Total RNA was isolated from HeLa cells according to the manufacturer’s instructions with the RNeasy Plus Mini Kit (Qiagen, Hilden, Germany). A QuantiTec Reverse Transcription Kit (Qiagen, Hilden, Germany) was used for reverse transcription of 1 µg of total RNA. qPCR was performed with the qTower (Analytik Jena, Jena, Germany) using the program 2 min 95 °C and 40 cycles of 10 s 95 °C and 20 s of 60 °C. The genes of interest were analyzed with EndoG-FAM, PolGA-FAM, and REV3L-FAM (Bio-Rad Laboratories, Hercules, CA, USA). As controls, PPIA-HEX, GAPDH-HEX (Bio-Rad Laboratories, Hercules, CA, USA), and B2M-VIC (Thermo Fisher Scientific, Waltham, MA, USA) were utilized.
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