Two microliters (1 μg) of each sample was injected onto a 0.075 × 500 mm EASY-Spray Pepmap C18 column equipped with a 0.100 × 5 mm EASY-Spray Pepmap C18 trap column (Thermo Fisher Scientific) attached to an EASY-nLC 1000 (Thermo Fisher Scientific). The peptides were separated using water (A) and acetonitrile (B) containing 0.1% formic acid as solvents at a flow rate of 300 nl per minute with a 3-h gradient. Data were acquired in positive ion data-dependent mode on an Orbitrap Fusion Lumos mass spectrometer (Thermo Fisher Scientific) with a resolution of 120,000 (at m/z 200) and a scan range from m/z 380 to 1500. Precursor isolation was performed using the quadrupole before either collision-induced dissociation activation in the ion trap and detection in the Orbitrap at a resolution of 30,000 or higher-energy collisional dissociation activation with detection in the Orbitrap at a resolution of 60,000.
Easy spray pepmap c18 trap column
Manufactured by Thermo Fisher Scientific
The EASY-Spray Pepmap C18 trap column is a solid-phase extraction (SPE) column designed for sample preparation in liquid chromatography-mass spectrometry (LC-MS) applications. The column is packed with a C18 stationary phase and is used to concentrate and purify peptide samples prior to analysis.
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Lab products found in correlation
2 protocols using easy spray pepmap c18 trap column
1
Peptide Separation and Mass Spectrometry Analysis
2
Liquid Chromatography-Mass Spectrometry Proteomics
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Two microliters (one microgram) of each sample was injected onto a 0.075 x 500 mm EASY-Spray Pepmap C18 column equipped with a 0.100 x 5 mm EASY-Spray Pepmap C18 trap column (Thermo-Fisher Scientific, San Jose, CA) attached to an EASY-nLC 1000 (Thermo-Fisher Scientific, San Jose, CA). The peptides were separated using water (A) and acetonitrile (B) containing 0.1% formic acid as solvents at a flow rate of 300 nL per minute with a three-hour gradient. Data were acquired in positive ion data-dependent mode on an Orbitrap Fusion Lumos mass spectrometer (Thermo-Fisher Scientific, San Jose, CA) with a resolution of 120,000 (at m/z 200) and a scan range from m/z 380-1500. Precursor isolation was performed using the quadrupole prior to either CID activation in the ion trap and detection in the Orbitrap at a resolution of 30,000 or HCD activation with detection in the Orbitrap at a resolution of 60,000.
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