Phosphatidylserine externalization was evaluated using the fluorescein isothiocyanate (FITC) Annexin V Apoptosis Detection kit (eBioscence; Thermo Fisher Scientific, Inc.). After treatment, cells were incubated with 25 µl/ml of Annexin V-FITC for 15 min at 37°C in a humidified atmosphere in the dark. Prior to flow cytometric analysis, propidium iodide (PI) was added to a final concentration of 5 µg/ml. Flow cytometric analysis was performed using a FACSCanto flow cytometer (Becton, Dickinson and Co.) equipped with 488 nm (blue) and 633 nm (red) lasers, and 10,000 events were recorded for each sample. Data acquisition and analysis were performed using FACSDiva software v.6.1.3. (Becton, Dickinson and Co.). In primary samples, Annexin V staining was combined with surface markers using the following antibodies: CD45-APC Vio770 (cat. no. 130-110-635), CD33-APC (cat. no. 130-111-020), CD14-PerCP Vio 700 (cat. no. 130-110-523), CD3-PE (cat. no. 130-113-139) (all from Miltenyi Biotec GmbH, dilution 1:50) and CD19-PE Cy7 (cat. no. 302216, 1:10, BioLegend, Inc.).
Cd19 pe cy7
Manufactured by Miltenyi Biotec
CD19-PE.cy7 is a fluorochrome-conjugated antibody that binds to the CD19 antigen. It is designed for use in flow cytometry applications to identify and quantify CD19-positive cells.
Automatically generated - may contain errors
Lab products found in correlation
Cd56 fitc, by Miltenyi Biotec (2 mentions)
Cd25 apc, by Miltenyi Biotec (2 mentions)
Cd107a pe cy5, by Miltenyi Biotec (2 mentions)
Cd3 pe, by Miltenyi Biotec (2 mentions)
Macsquant flow cytometry, by Miltenyi Biotec (2 mentions)
Cd8 vioblue, by Miltenyi Biotec (2 mentions)
Cd4 viogreen, by Miltenyi Biotec (2 mentions)
Ficoll paque plus, by GE Healthcare (2 mentions)
Facscanto flow cytometer, by BD (1 mentions)
3 protocols using cd19 pe cy7
1
Evaluating Phosphatidylserine Externalization
2
Dornase alfa and MgSO4 Effects on PBMC
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Three healthy adult blood was obtained from Acibadem Labcell Cellular Therapy Laboratory.
Following the isolation of peripheral mononuclear cells (PBMC) by overlaying blood on Ficollpaque plus (GE Healthcare), the serially diluted (300 U/mL, 100 U/mL, 30 U/mL, 10 U/mL, 3 U/mL) Dornase alfa (Pulmozyme®, Roche, USA) and 420 g/mL MgSO 4 (Magnesium Sulphate 15% Onfarma, TR) were incubated with the PBMCs for 48hr in T cell medium (6% Human AB Serum and 1% Pen/Strep, Texmacs medium). Immune cell subtypes and their activation levels were determined by Miltenyi MACSQuant flow cytometry analysis using CD3-PE, CD19-PE.cy7, CD56-FITC, CD4-Viogreen, CD8-Vioblue, CD25-APC and CD107a-PE.cy5.5 (Miltenyi).
Following the isolation of peripheral mononuclear cells (PBMC) by overlaying blood on Ficollpaque plus (GE Healthcare), the serially diluted (300 U/mL, 100 U/mL, 30 U/mL, 10 U/mL, 3 U/mL) Dornase alfa (Pulmozyme®, Roche, USA) and 420 g/mL MgSO 4 (Magnesium Sulphate 15% Onfarma, TR) were incubated with the PBMCs for 48hr in T cell medium (6% Human AB Serum and 1% Pen/Strep, Texmacs medium). Immune cell subtypes and their activation levels were determined by Miltenyi MACSQuant flow cytometry analysis using CD3-PE, CD19-PE.cy7, CD56-FITC, CD4-Viogreen, CD8-Vioblue, CD25-APC and CD107a-PE.cy5.5 (Miltenyi).
3
Dornase alfa and MgSO4 Effects on PBMC
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Three healthy adult blood was obtained from Acibadem Labcell Cellular Therapy Laboratory.
Following the isolation of peripheral mononuclear cells (PBMC) by overlaying blood on Ficollpaque plus (GE Healthcare), the serially diluted (300 U/mL, 100 U/mL, 30 U/mL, 10 U/mL, 3 U/mL) Dornase alfa (Pulmozyme®, Roche, USA) and 420 g/mL MgSO 4 (Magnesium Sulphate 15% Onfarma, TR) were incubated with the PBMCs for 48hr in T cell medium (6% Human AB Serum and 1% Pen/Strep, Texmacs medium). Immune cell subtypes and their activation levels were determined by Miltenyi MACSQuant flow cytometry analysis using CD3-PE, CD19-PE.cy7, CD56-FITC, CD4-Viogreen, CD8-Vioblue, CD25-APC and CD107a-PE.cy5.5 (Miltenyi).
Following the isolation of peripheral mononuclear cells (PBMC) by overlaying blood on Ficollpaque plus (GE Healthcare), the serially diluted (300 U/mL, 100 U/mL, 30 U/mL, 10 U/mL, 3 U/mL) Dornase alfa (Pulmozyme®, Roche, USA) and 420 g/mL MgSO 4 (Magnesium Sulphate 15% Onfarma, TR) were incubated with the PBMCs for 48hr in T cell medium (6% Human AB Serum and 1% Pen/Strep, Texmacs medium). Immune cell subtypes and their activation levels were determined by Miltenyi MACSQuant flow cytometry analysis using CD3-PE, CD19-PE.cy7, CD56-FITC, CD4-Viogreen, CD8-Vioblue, CD25-APC and CD107a-PE.cy5.5 (Miltenyi).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!