Nexera x2 uhplc lc 30a system

The UV spectrum and data of absorbance were determined by using a HITACHI U-3010 spectrophotometer (Hitachi Ltd., Tokyo, Japan). The IR spectrum was obtained by using an IR200 spectrophotometer (Thermo Electron Corporation, Waltham, MA). The Circular dichroism spectra were obtained using J-810 Circular Dichroism spectrometer (JASCO Corporation, Japan). Optical rotations were measured with Autopol I automatic polarmeter (Rudolph Research Analytical, Hackettstown, NJ). The NMR spectra were recorded using an AVANCE 500 MHz instrument (Bruker Corporation, Switzerland). All compounds were dissolved in DMSO-d 6 or CDCl 3. Relative molecular mass were recorded by using a 6520 quadrupole-time-of-flight mass spectrometer (Agilent, Palo Alto, CA). Purity was detected on a Nexera X2 UHPLC LC-30A system (Shimadzu, Kyoto, Japan), using a Kromasil C18 column (150 mm × 4.6 mm, 5 μm, Dalian Johnsson Separation Science and Technology Corporation). The oven temperature was maintained at 40 °C. Water containing 0.1% formic acid served as solvent system A, and methanol served as solvent system B. The flow rate was 1.0 mL/min, and injection volume was 5 μL. 96-well microplate reader (HBS-1096A) was purchased from Nanjing Detie Experimental Equipment Co., Ltd. (Nanjing, China).