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Nexera x2 uhplc lc 30a system

Manufactured by Shimadzu
Sourced in Japan

The Nexera X2 UHPLC LC-30A system is a high-performance liquid chromatography (HPLC) instrument designed for analytical applications. It features a modular architecture that includes a solvent delivery unit, an autosampler, a column oven, and a UV/Vis detector. The system is capable of operating at ultra-high pressures, enabling the use of smaller particle size columns for improved separation efficiency and reduced analysis time.

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2 protocols using nexera x2 uhplc lc 30a system

1

NMR and Mass Spectrometry Analysis of Compounds

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The NMR spectra were recorded using an AVANCE 600 MHz instrument (Bruker Corporation, Switzerland). The compounds were dissolved in DMSO-d 6 . Molecular mass was recorded by using a 6520 quadrupole-time-of-flight mass spectrometer (Agilent, Palo Alto, CA). In the separation process, column chromatography (CC) included silica gel (100-220 and 200-300 mesh, Qingdao Marine Chemical Co., Qingdao, China), a polyamide resin (80-100 mesh, Taizhou Luqiao Sijia Biochemical Plastic Factory, Zhejiang, China) and ODS (20-40 μm, GE Healthcare, Marlborough, MA). TLC was performed on silica gel GF254 (Qingdao Marine Chemical Co., Qingdao, China). A final purification step was conducted with a Shimadzu Nexera X2 UHPLC LC-30A system (Shimadzu, Kyoto, Japan) equipped with a Kromasil C18 (4.6 × 150 mm, 5 µm) column (Dalian, China).
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2

Comprehensive Analytical Techniques for Compound Characterization

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The UV spectrum and data of absorbance were determined by using a HITACHI U-3010 spectrophotometer (Hitachi Ltd., Tokyo, Japan). The IR spectrum was obtained by using an IR200 spectrophotometer (Thermo Electron Corporation, Waltham, MA). The Circular dichroism spectra were obtained using J-810 Circular Dichroism spectrometer (JASCO Corporation, Japan). Optical rotations were measured with Autopol I automatic polarmeter (Rudolph Research Analytical, Hackettstown, NJ). The NMR spectra were recorded using an AVANCE 500 MHz instrument (Bruker Corporation, Switzerland). All compounds were dissolved in DMSO-d 6 or CDCl 3. Relative molecular mass were recorded by using a 6520 quadrupole-time-of-flight mass spectrometer (Agilent, Palo Alto, CA). Purity was detected on a Nexera X2 UHPLC LC-30A system (Shimadzu, Kyoto, Japan), using a Kromasil C18 column (150 mm × 4.6 mm, 5 μm, Dalian Johnsson Separation Science and Technology Corporation). The oven temperature was maintained at 40 °C. Water containing 0.1% formic acid served as solvent system A, and methanol served as solvent system B. The flow rate was 1.0 mL/min, and injection volume was 5 μL. 96-well microplate reader (HBS-1096A) was purchased from Nanjing Detie Experimental Equipment Co., Ltd. (Nanjing, China).
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