Mice were anesthetized with 2% isoflurane in oxygen, and an osmotic mini-pump (Alzet micro-osmotic pump model 1007D; Alzet DURECT Corporation, Cupertino, CA, USA) was implanted subcutaneously between the scapulae. Pumps were filled with AngII in sterile saline and were set to deliver AngII (200 ng/kg per/min). Control mice received infusion of saline of comparable volume. WT and Sirt3-KO mice were randomly assigned to the control group or Ang II-treated group. Animals were sacrificed four weeks after surgery and their hearts were removed to be analyzed for the development of myocardial hypertrophy and autophagy flux. Mini-pumps were weighed afterwards in order to verify complete diffusion.
Alzet micro osmotic pump model 1007d
Manufactured by Durect
Sourced in United States
The Alzet micro-osmotic pump model 1007D is a laboratory equipment designed to deliver small, controlled amounts of substances continuously over an extended period of time. It operates on the principle of osmosis, using the natural movement of water across a semipermeable membrane to provide the pumping action. The pump has a volume of 0.5 milliliters and is capable of delivering at a rate of 0.5 microliters per hour for up to 7 days.
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Lab products found in correlation
2 protocols using alzet micro osmotic pump model 1007d
1
Angiotensin II-Induced Cardiac Hypertrophy
2
Systemic Inflammation via Intraperitoneal LPS Infusion
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Regarding the intraperitoneal infusion (IP), systemic inflammation was induced by continuous intraperitoneal infusion via an osmotic minipump of LPS (5 ng•100 g-1•hour-1 for 7 days; Sigma–Aldrich, St. Louis, MO, USA). On the day of implantation, animals were anesthetized with sodium pentobarbital (50 mg/kg, IP), and osmotic minipumps (ALZET® Micro-Osmotic Pump Model 1007D; Durect, Cupertino, CA, USA) were placed in the peritoneal cavity. Control animals received saline-filled osmotic minipumps, and the sham-operated animals underwent identical surgical procedures with the implantation of a vehicle-filled osmotic minipump. Following implantation, the abdominal muscles were closed in layers, and body temperature was maintained at 37℃ with a heating pad until the animals recovered from anesthesia.
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