3 4 5 dimethylthiazol 2 yl 2 5 diphenyltetrazolium bromide mtt

METH was provided by Ningbo Public Security Bureau. N-Acetyl-L-cysteine (NAC) was purchased from Sigma-Aldrich. All drugs were dissolved in saline (0.9% NaCl) and administered to cells directly or via intraperitoneal injection. 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) and DMSO were purchased from MedChemExpress. The BrdU cell proliferation kit was purchased from BioVision. The primary and secondary antibodies for Western blotting, including anti-Ras, anti-β-actin, anti-ERK, anti-pERK, anti-MEK, and anti-pMEK, were purchased from Proteintech. Polyvinylidene fluoride, blocking buffer, and ECL were purchased from Millipore. The Active Ras Pull-Down and Detection Kit was purchased from Thermo Fisher Scientific.

Glyceryl monostearate, medium chain triglycerides and poloxamer 188 were purchased from AIKE REAGENT Co., Ltd. (Chengdu, China). Absolute ethanol was purchased from the DAMAO Chemical Reagent Factory (Tianjin, China). Phosphoric acid and methanol were supplied by FUYU CHEMICAL (Tianjin, China). TMZ and CUR were purchased from Aladdin Co., Ltd. (Shanghai, China). 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) was purchased from Med Chem Express Co., Ltd. (New Jersey, USA). Hoechst 33342 Live Cell Staining Solution was obtained from Beyotime Co., Ltd. (Shanghai, China). All other chemical compounds were of analytical purity. Rat glioma cell line (C6) and human brain glial normal cell line (HEB) were obtained from Jennio Biotech Co., Ltd. (Guangzhou, China).