Coverslips were fixed in 4% paraformaldehyde overnight at 4° C and blocked with 10% Normal Goat Serum (NGS) followed by anti-TOM20 antibody (1:100 dilution, Santa Cruz Biotechnology, Dallas, TX, USA) or DLP1 (1:500 dilution, BD Transduction 611112, San Jose, CA, USA) and AlexaFluor 488 goat anti-rabbit or goat anti-mouse antibody (1:100 dilution, Invitrogen Molecular Probes, Eugene, OR, USA). Slides were viewed on a Fluoview (FV10i) Olympus Confocal Microscope (Olympus America Inc., Melville, NY, USA) with a 60× UPLSAP60xW objective with 1.0 aperture and 3× optical zoom at room temperature.
Uplsap60xw objective
Manufactured by Olympus
Sourced in United States
The 60× UPLSAP60xW objective is a high-performance microscope objective lens designed for use in a variety of scientific applications. It offers a magnification of 60x and is optimized for wide-field observations. The objective lens features a numerical aperture of 1.20 and a working distance of 0.15 mm, providing high-resolution imaging capabilities.
Automatically generated - may contain errors
2 protocols using uplsap60xw objective
1
Mitochondrial Dynamics Visualization
2
Immunofluorescence Imaging of Mitochondrial Dynamics
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Coverslips were fixed in 4% paraformaldehyde overnight at 4°C and
blocked with 10% Normal Goat Serum (NGS) followed by anti-TOM20 antibody
(1:100 dilution, Santa Cruz Biotechnology, Dallas, TX, USA) or DLP1 (1:500
dilution, BD Transduction 611112, San Jose, CA, USA) and AlexaFluor 488 goat
anti-rabbit or goat anti-mouse antibody (1:100 dilution, Invitrogen Molecular
Probes, Eugene, OR, USA). Slides were viewed on a Fluoview (FV10i) Olympus
Confocal Microscope (Olympus America Inc., Melville, NY, USA) with a 60×
UPLSAP60xW objective with 1.0 aperture and 3× optical zoom at room
temperature.
blocked with 10% Normal Goat Serum (NGS) followed by anti-TOM20 antibody
(1:100 dilution, Santa Cruz Biotechnology, Dallas, TX, USA) or DLP1 (1:500
dilution, BD Transduction 611112, San Jose, CA, USA) and AlexaFluor 488 goat
anti-rabbit or goat anti-mouse antibody (1:100 dilution, Invitrogen Molecular
Probes, Eugene, OR, USA). Slides were viewed on a Fluoview (FV10i) Olympus
Confocal Microscope (Olympus America Inc., Melville, NY, USA) with a 60×
UPLSAP60xW objective with 1.0 aperture and 3× optical zoom at room
temperature.
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