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In situ death detection tunel kit

Manufactured by Roche
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The In Situ Death Detection TUNEL Kit is a laboratory equipment product designed to detect and visualize DNA fragmentation, a hallmark of apoptosis or programmed cell death. The kit utilizes the TUNEL (Terminal deoxynucleotidyl transferase dUTP Nick End Labeling) method to label the free 3'-hydroxyl termini in DNA strand breaks. This allows for the identification and quantification of cells undergoing apoptosis.

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Lab products found in correlation

Lsm 710 confocal microscope, by Zeiss (6 mentions) A1 confocal microscope, by Nikon (2 mentions) Dnase 1, by Roche (2 mentions) Histostain kit, by Thermo Fisher Scientific (2 mentions) Dab substrate, by Thermo Fisher Scientific (2 mentions) Ire1α, by Cell Signaling Technology (2 mentions)

Spelling variants of this product

TUNEL kit (509 citations) TUNEL detection kit (108 citations) TUNEL in situ cell death detection kit (67 citations) In Situ Death Detection Kit (21 citations) In Situ Cell Death Detection Kit (TUNEL fluorescence FITC kit; (7 citations) Fluorescein-conjugated TUNEL in situ cell death detection kit (5 citations) TUNEL staining in situ cell death detection kit (4 citations) TUNEL in situ cell death detection kit AP (4 citations) In Situ Cell Death detection kit (TUNEL assay) (3 citations) TUNEL in situ cell death detection kit-POD (2 citations)

4 protocols using in situ death detection tunel kit

1

Quantifying Apoptosis in Pancreatic Tissue

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TUNEL staining was performed as previously described2 (link). Briefly, paraffin-embedded pancreatic sections were rehydrated, treated with protease K, and stained for 1 hour at 37°C using the in Situ Death Detection TUNEL Kit (Roche 11684795910). For positive control, slides were treated with DNase I (Roche) for 30 minutes at room temperature. Fluorescence Images were captured under a Nikon A1 confocal microscope at Brehm Diabetes Research Center Imaging Facility at the University of Michigan.
Ji Y., Sun S., Shrestha N., Darragh L.B., Shirakawa J., Xing Y., He Y., Carboneau B.A., Kim H., An D., Ma M., Oberhozler J., Soleimanpour S.A., Gannon M., Liu C., Naji A., Kulkarni R.N., Wang Y., Kersten S, & Qi L. (2019). Toll-like receptors TLR2 and TLR4 block the replication of pancreatic β cells in diet-induced obesity. Nature immunology, 20(6), 677-686.
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2

Immunohistochemistry and TUNEL Staining of Intestinal Tissue

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Formalin-fixed, paraffin-embedded intestinal sections were processed for IHC and TUNEL staining as previously described10 (link). IHC was performed with the Histostain kit and DAB substrate from Invitrogen. Primary antibodies used for IHC were IRE1α (#3294, 1:100) from Cell Signaling and OS9 (ab109510, 1:200) from Abcam. Sections were scanned and images were taken using the Aperio Scanscope. TUNEL staining was performed with the in Situ Death Detection TUNEL Kit (Roche 11684795910). The fluorescence was visualized and images were captured under a Zeiss LSM710 confocal microscope with 10× magnification at Cornell Biotechnology Resource Center Imaging Facility.
Sun S., Shi G., Sha H., Ji Y., Han X., Shu X., Ma H., Inoue T., Gao B., Kim H., Bu P., Guber R.D., Shen X., Lee A.H., Iwawaki T., Paton A.W., Paton J.C., Fang D., Tsai B., Yates JR I.I.I., Wu H., Kersten S., Long Q., Duhamel G.E., Simpson K.W, & Qi L. (2015). IRE1α is an endogenous substrate of endoplasmic reticulum-associated degradation. Nature cell biology, 17(12), 1546-1555.
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3

Immunohistochemistry and TUNEL Staining of Intestinal Tissue

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Formalin-fixed, paraffin-embedded intestinal sections were processed for IHC and TUNEL staining as previously described10 (link). IHC was performed with the Histostain kit and DAB substrate from Invitrogen. Primary antibodies used for IHC were IRE1α (#3294, 1:100) from Cell Signaling and OS9 (ab109510, 1:200) from Abcam. Sections were scanned and images were taken using the Aperio Scanscope. TUNEL staining was performed with the in Situ Death Detection TUNEL Kit (Roche 11684795910). The fluorescence was visualized and images were captured under a Zeiss LSM710 confocal microscope with 10× magnification at Cornell Biotechnology Resource Center Imaging Facility.
Sun S., Shi G., Sha H., Ji Y., Han X., Shu X., Ma H., Inoue T., Gao B., Kim H., Bu P., Guber R.D., Shen X., Lee A.H., Iwawaki T., Paton A.W., Paton J.C., Fang D., Tsai B., Yates JR I.I.I., Wu H., Kersten S., Long Q., Duhamel G.E., Simpson K.W, & Qi L. (2015). IRE1α is an endogenous substrate of endoplasmic reticulum-associated degradation. Nature cell biology, 17(12), 1546-1555.
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4

Quantifying Apoptosis in Pancreatic Tissue

Check if the same lab product or an alternative is used in the 5 most similar protocols
TUNEL staining was performed as previously described2 (link). Briefly, paraffin-embedded pancreatic sections were rehydrated, treated with protease K, and stained for 1 hour at 37°C using the in Situ Death Detection TUNEL Kit (Roche 11684795910). For positive control, slides were treated with DNase I (Roche) for 30 minutes at room temperature. Fluorescence Images were captured under a Nikon A1 confocal microscope at Brehm Diabetes Research Center Imaging Facility at the University of Michigan.
Ji Y., Sun S., Shrestha N., Darragh L.B., Shirakawa J., Xing Y., He Y., Carboneau B.A., Kim H., An D., Ma M., Oberhozler J., Soleimanpour S.A., Gannon M., Liu C., Naji A., Kulkarni R.N., Wang Y., Kersten S, & Qi L. (2019). Toll-like receptors TLR2 and TLR4 block the replication of pancreatic β cells in diet-induced obesity. Nature immunology, 20(6), 677-686.
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