Acrylamide (AM), 4-vinylbenzenesulfonic acid sodium salt hydrate (SSNa), Tween85, Tween60 and cetyltrimethyl ammonium bromide (CTAB) were provided by Adamas Reagent Co., Ltd. N,N′-Methylene bisacrylamide (MBAM) was purchased from Sigma-Aldrich. 2,4,6-Trimethylbenzoyldiphenyl phosphine oxide (TPO), cyclohexane and ethanol were brought from Titan Scientific Co., Ltd (Shanghai, China). Liquid paraffin was obtained from Lingfeng Chemical Reagents Co. Ltd (Shanghai, China). All the reagents were used as received. Deionized water was used in this work.
N n methylene bisacrylamide mbam
Manufactured by Merck Group
Sourced in United States
N,N′-Methylene bisacrylamide (MBAM) is a chemical compound used as a cross-linking agent in various laboratory applications. It is a white crystalline solid that is soluble in water and other polar solvents. MBAM is commonly used in the preparation of polyacrylamide gels, which are widely employed in techniques such as electrophoresis and gel chromatography.
Automatically generated - may contain errors
Lab products found in correlation
Acrylamide aam, by Merck Group (2 mentions)
Cetyltrimethyl ammonium bromide, by Adamas (1 mentions)
Anhydrous sodium acetate, by Merck Group (1 mentions)
Ammonium persulphate, by Merck Group (1 mentions)
Tetramethylethyldiamide, by Merck Group (1 mentions)
Fluorescein o acrylate, by Merck Group (1 mentions)
Phosphate buffered saline (pbs), by Merck Group (1 mentions)
Acetonitrile, by Merck Group (1 mentions)
Sodium dodecyl sulfate (sds), by Merck Group (1 mentions)
Bovine serum albumin (bsa), by Merck Group (1 mentions)
Glacial acetic acid, by Merck Group (1 mentions)
Ethyl acetate, by Merck Group (1 mentions)
Acrylamide, by Merck Group (1 mentions)
Ethylene glycol, by Merck Group (1 mentions)
2 hydroxy 1 4 hydroxyethoxy phenyl 2 methyl 1 propanone, by Merck Group (1 mentions)
Benzophenone, by Merck Group (1 mentions)
2 hydroxy 2 methylpropiophenone irgacure 1173, by Merck Group (1 mentions)
3 trimethoxysilyl propyl methacrylate γ maps, by Merck Group (1 mentions)
Toluene, by Chem-Supply (1 mentions)
Methanol, by Merck Group (1 mentions)
5 protocols using n n methylene bisacrylamide mbam
1
Fabrication of Polymeric Microparticles
2
Fluorescent Polymer Synthesis Protocol
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7-[4-(Trifluoromethyl)coumarin]methacrylamide), acetonitrile, acrylamide (AAm),
ammonium persulphate (APS), anhydrous sodium acetate (NaOAc), bovine serum albumin
(BSA) ethanol, ethyl acetate, ethylene glycol, ferric chloride hexahydrate
(FeCl3 · 6H2O), fetal calf serum (FCS), fluorescein O-acrylate, glacial acetic acid (AcOH), glutaraldehyde,
myoglobin (Mb) (from equine skeletal muscle), N N,
N′-methylenebisacrylamide (mBAm), phosphate buffered saline (PBS), sodium
dodecyl sulfate (SDS), tetramethylethyldiamide (TEMED), were all purchased and used
without purification from Sigma-Aldrich, Poole, Dorset, UK.
ammonium persulphate (APS), anhydrous sodium acetate (NaOAc), bovine serum albumin
(BSA) ethanol, ethyl acetate, ethylene glycol, ferric chloride hexahydrate
(FeCl3 · 6H2O), fetal calf serum (FCS), fluorescein O-acrylate, glacial acetic acid (AcOH), glutaraldehyde,
myoglobin (Mb) (from equine skeletal muscle), N N,
N′-methylenebisacrylamide (mBAm), phosphate buffered saline (PBS), sodium
dodecyl sulfate (SDS), tetramethylethyldiamide (TEMED), were all purchased and used
without purification from Sigma-Aldrich, Poole, Dorset, UK.
3
Photocrosslinkable PNIPAAm Hydrogel Synthesis
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Photocrosslinkable PNIPAAm was synthesized using the free-radical polymerization method. 100mg of N-isopropylacrylmide monomer (NIPAAm) (Sigma Aldrich, USA) was dissolved in 0.6 ml of deionized water to preparing a pre-gel solution. In this monomer solution, 1mg of 2-hydroxy-1-[4-(hydroxyethoxy)phenyl]-2methyl-1-propanone (Sigma Aldrich, Iragacure 2959, USA) was added as a photoinitiator. In this study, two different concentrations of crosslinker (C1 and C3) were use: 1 mg and 3 mg of N,N’-methylenebisacrylamide (MBAm) (Sigma Aldrich, USA) were dissolved in 0.1 ml of deionized water.
The 700 μl pre-gel solution was poured into a Polydimethylsiloxane (PDMS) (Dow Corning Corp., USA) mould. Then, 1mm supporting beams were installed along the PDMS mould wall for height equalization. The solution covered with a film was irradiated for 8 min with 365nm UV light (VIRVER Lourmat-4.L, France). In the illuminated regions, free radicals leads to polymerization.
The 700 μl pre-gel solution was poured into a Polydimethylsiloxane (PDMS) (Dow Corning Corp., USA) mould. Then, 1mm supporting beams were installed along the PDMS mould wall for height equalization. The solution covered with a film was irradiated for 8 min with 365nm UV light (VIRVER Lourmat-4.L, France). In the illuminated regions, free radicals leads to polymerization.
4
Polyacrylamide Hydrogel Fabrication
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Poly(N-isopropylacrylamide) (PNIPAAm; Sigma–Aldrich A3553; Mn ~40,000, Sigma–Aldrich, St. Louis, MO, USA), acrylamide (AAm; Sigma–Aldrich A3553; ≥99%), 2-hydroxy-2-methylpropiophenone (Irgacure 1173; Sigma–Aldrich 405655; 97%), N, N′-Methylenebisacrylamide (MBAM; Sigma–Aldrich M7279; ≥99.5%) and benzophenone (Sigma–Aldrich B9300; ≥ 99%) were purchased from Sigma Aldrich. Polydimethylsiloxane (PDMS; Dow Corning Sylgard 184 Silicone Elastomer Kit, Dow Corning, Midland, MI, USA) was purchased from Dow Corning. PAAm hydrogel layer was molded from a Teflon mold (65 mm × 40 mm × 0.1 mm (length × width × thickness)).
5
Synthesis of RAFT-Mediated Polymer Hydrogels
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Poly (ethylene glycol) methyl ether acrylate (PEO MA, average Mn ≈ 480) was purchased from Sigma-Aldrich and used as received. Styrene (Sty, Aldrich, 99%) was passed through a column of Al2O3 to remove the inhibitor. The RAFT agent, 2-[[(butylsulfanyl)-carbonothioyl]sulfanyl] propanoic acid (PABTC), was synthesized as described in ref. 60 (link). 4,4′-azobis(4-cyanovaleric acid) (V501, >98%, Aldrich) was used as received. Acrylamide (AAM, Sigma-Aldrich, ≥98%), N,N′-methylenebisacrylamide (MBAM, Sigma-Aldrich, ≥99.5%)), methanol (Fluka), basic alumina (Al2O3, Brockman activity I, 60–325 mesh), N,N,N′,N′- tetramethylethylenediamine (TEMED, Sigma-Aldrich, 99%), were all used as received. 3-(trimethoxysilyl)propyl methacrylate (γ -MAPS) was obtained from Sigma (St. Louis, MO, USA). Toluene was obtained from Chem-Supply (Gillman, SA, AUS). Potassium persulfate (KPS, M&B, 98%) was recrystallized from water.
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