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Ptenflox mice

Manufactured by Jackson ImmunoResearch
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The Ptenflox mice are a genetically modified mouse model developed by Jackson ImmunoResearch. The mice have a conditional deletion of the Pten gene, which is known to be a tumor suppressor. This model can be used to study the role of the Pten gene in various biological processes and disease states.

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Lab products found in correlation

Cd11c dtr, by Jackson ImmunoResearch (1 mentions) Flt3lko mice, by Taconic Biosciences (1 mentions) Foxp3dtr, by Jackson ImmunoResearch (1 mentions) Cd11c cre, by Jackson ImmunoResearch (1 mentions) C57bl 6, by Jackson ImmunoResearch (1 mentions) Pdgfrαflox, by Jackson ImmunoResearch (1 mentions) Pdgfrαgfp, by Jackson ImmunoResearch (1 mentions)

3 protocols using ptenflox mice

1

Mice Genotypes and Sourcing

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Six week-old female Flt3LKO mice were purchased from Taconic. C57BL/6, CD11c-DTR, CD11c-Cre, Foxp3-DTR and Pten-flox mice were purchased from the Jackson Laboratory. Flk2KO mice were kindly provided by Ihor Lemischka from Ichan School of Medicine at Mount Sinai. Batf3KO mice were gifts from Kenneth Murphy from Washington University. DC-specific Pten knockout mice (DCΔPTEN) were generated by breeding CD11c-Cre with Pten-flox mice. Cre-negative littermates (Pten-Flox) were used as control. All procedures were in accordance with Institutional Animal Care and Use Committee Protocols.
Jiao J., Dragomir A.C., Kocabayoglu P., Rahman A.H., Chow A., Hashimoto D., Leboeuf M., Kraus T., Moran T., Carrasco-Avino G., Friedman S.L., Merad M, & Aloman C. (2014). Central Role of Conventional Dendritic Cells in Regulation of Bone Marrow Release and Survival of Neutrophils. Journal of immunology (Baltimore, Md. : 1950), 192(7), 3374-3382.
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2

Genetic Mouse Models for Transforming Growth Factor-Beta Signaling

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Mice were on a mixed C57BL/6/129 genetic background and the use of mice for this study was approved by the Institutional Animal Care and Use Committee at Texas A&M University. The Pgr-Cre and Tgfbr1flox mice were generated previously [19 (link),20 (link)]. The Pgr-Cre mice were obtained from Drs. John Lydon and Francesco DeMayo, and the Tgfbr1flox mice were contributed by Dr. Stefan Karlsson and imported from the Matzuk laboratory at Baylor College of Medicine. The Ptenflox mice were purchased from The Jackson Laboratory (stock # 006440; Bar Harbor, ME, USA) [21 (link)]. The genotypes of mice and DNA recombination were analyzed by genomic PCR (supplementary material, Table S1) [20 (link)-29 (link)].
Gao Y., Lin P., Lydon J.P, & Li Q. (2017). Conditional abrogation of Transforming Growth Factor Beta Receptor 1 in PTEN-inactivated endometrium promotes endometrial cancer progression in mice. The Journal of pathology, 243(1), 89-99.
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3

Genetic Manipulation of Mice for Research

Cited 1 time
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Mice carrying Frs2αflox and Shp2flox alleles were bred and genotyped as described [67 (link), 68 (link)]. We obtained Fgf3OVE391 from Dr. Michael Robinson (Miami University, Oxford, OH, USA), Le-Cre from Drs. Ruth Ashery-Padan (Tel Aviv University, Tel Aviv, Israel) and Richard Lang (Children's Hospital Research Foundation, Cincinnati, OH, USA), p85αflox and p85βKO from Dr. Lewis Cantley (Weill Cornell Medicine, New York, NY, USA), and PdgfrαΔPI3K from Dr. Philipo Soriano (Mount Sinai School of Medicine, New York, NY, USA) [16 (link), 20 (link), 25 (link), 26 (link), 46 (link), 69 (link)]. Pdgfrαflox (stock no.: 006492), PdgfrαGFP (stock no.: 007669), and Ptenflox mice (stock no.: 006440) and Rosa-N1-ICDflox/+ (stock no.: 008159) and Baxflox/flox;BakKO/KO (stock no.: 006329) mice were obtained from Jackson Laboratory (Bar Harbor, ME, USA) [31 (link), 32 (link), 43 (link), 70 (link)–72 (link)]. In all conditional KO experiments, mice were maintained on a mixed genetic background, and Le-Cre–only or Le-Cre and heterozygous flox mice were used as controls. Mouse maintenance and experimentation were performed according to protocols approved by Columbia University Institutional Animal Care and Use Committee.
Li H., Mao Y., Bouaziz M., Yu H., Qu X., Wang F., Feng G.S., Shawber C, & Zhang X. (2019). Lens differentiation is controlled by the balance between PDGF and FGF signaling. PLoS Biology, 17(2), e3000133.
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