Anti ctbp1

After separation in SDS-PAGE (AA: 10%, AA/BisAA ratio: 36:1), the proteins were transferred onto PVDF membranes following overnight incubation with specific primary antibodies. The following primary antibodies were used: anti-actin (Sigma A5441, Sigma-Aldrich, St. Louis, MO, USA), anti-Zeb1 (Sigma AMAb90510, Sigma-Aldrich, St. Louis, MO, USA), anti-Nanog (Santa Cruz sc-293121, Dallas, TX, USA), anti-Oct4 (Cell Signaling #2890s, Danvers, MA, USA), anti-Sox2 (Cell Signaling #3579s, Danvers, MA, USA), anti-CTBP2 (Abcam ab128871, Cambridge, UK), anti-CTBP1 (Sigma HPA018987, Sigma-Aldrich, St. Louis, MO, USA), anti-LSD1 (Sigma ABE365, Sigma-Aldrich, St. Louis, MO, USA), anti-TRIM33 (Sigma HPA004345, Sigma-Aldrich, St. Louis, MO, USA), and anti-p53 (Cell Signaling #46565, Danvers, MA, USA). The secondary antibodies were from Sigma: anti-mouse (A9917) and anti-rabbit (A0545). Bound antibodies were visualized using the SuperSignal West Femto Maximum Sensitivity Substrate ECL kit (Thermo scientific, Waltham, MA, USA), chemiluminescence was detected using ChemiDoc (BioRad, Hercules, CA, USA).