Cholic acid

Quercetin 7-rhamnoside was purchased from Biopurify (Chengdu Biopurify Phytochemicals Ltd.). Kits for detecting albumin (ALB), aspartate aminotransferase (AST), and alanine aminotransferase (ALT) were purchased from Nanjing Jiancheng (Nanjing Jiancheng Bioengineering Institute). UrsodeoxyCholic acid (>98%, J0324A), ChenodeoxyCholic acid (>98%, D1217A), Cholic acid (>98%, M0412AS), HyodeoxyCholic acid (>98%, D1017AS), DeoxyCholic acid (>98%, J0602AS) were purchased from meilunbio (Dalian Meilun Biotech Co., Ltd.). d4-glycochenodeoxycholate (>99.5%, ZZS19022701) was purchased from ZZBIO (Shanghai ZZBIO Co., Ltd.). 5-(Diisopropylamino) amylamine (DIAAA, B02669513), 1-[Bis (dimethylamino) methylene]-1H-1,2,3-triazolo [4,5-b] pyridinium 3-oxid hexafluorophosphate (HATU,≥98.0%,102409029), 1-Hydroxybenzotriazole hydrate (HOBt, ≥97.0%, BCCF3867), Triethylamine (TEA, ≥99.5%) were purchased from Sigma (Sigma-Aldrich (Shanghai) Trading Co. Ltd.). Primary anti-FXR antibodies were purchased from Abcam (Abcam Plc.). anti-CYP7A1 antibodies were purchased from Boster (Boster Biological Technology Co., Ltd.), and anti-CYP27A1 antibodies were purchased from Affinity Biosciences (Affinity Biosciences Co., Ltd.). Secondary antibodies such as goat anti-mouse IgG (H + L) and goat anti-rabbit IgG (H + L) were purchased from CWBIO (Beijing Kangwei Century Biotechnology Co., Ltd.).

One gram of BBD (Xiamen Chinese Medicine Factory, 160730) was dissolved in 20 mL deionized water, ultrasonicated, and centrifugated at 2500 rpm for 5 min. Precipitate was dissolved in 20 mL of 70% ethanol, ultrasonicated, and centrifugated at 2500 rpm for 5 min. The total soluble solid in the 70% ethanol extract was determined and resolved in DMSO (SINOPHARM, 30072418) at a concentration of 5 mg/mL. C. bovis (Xiamen Chinese Medicine Factory) was extracted twice with a 10-fold volume of chloroform-methanol (1:1) for 1 h. The total soluble solid was obtained by centrifugation and concentration, followed by resolving in DMSO at a concentration of 20 mg/mL. Bile acids were dissolved in DMSO at a concentration of 10 mg/mL, including Taurodeoxycholate sodium salt (TDCA, DN0032), DeoxyCholic acid (DCA, DQ0014), TauroCholic acid Sodium Salt (TCA, DN0030), GlycodeoxyCholic acid(GDCA) purchased form Desite (Chengdu, China), GlycoCholic acid (GCA, MB5234), Cholic acid (CA, MB5177) purchased form meilunbio (Dalian, China), TaurochenodeoxyCholic acid (TCDCA, B20919), GlycochenodeoxyCholic acid(GCDCA, B24724), dexamethasone (DEX, B25793) purchased from Yuanye Bio-Technology (Shanghai, China)