Jatrorrhizine

Manufactured by Yuanye Bio-Technology

Sourced in China

Jatrorrhizine is a laboratory chemical compound used in scientific research. It is a natural alkaloid extracted from certain plant species. Jatrorrhizine can be used as a reference standard or as a starting material for the synthesis of other compounds. The core function of Jatrorrhizine is to serve as a research tool for chemical and biological investigations, but no further details on its intended use can be provided in an unbiased and factual manner.

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5 protocols using jatrorrhizine

Extraction and Analysis of Bioactive Compounds

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S. acutum was purchased from Anhui FengyuanTongling Chinese Herbal Medicine Co., Ltd. (Anhui, China). Standards of higenamine, sinomenine, magnoflorine, tetrahydroberberine, tetrahydro palmatine, berberrubine, jatrorrhizine, columbamine, berberine, and palmatine were purchased from the Shanghai Yuanye Biological Technology Co., Ltd. (Shanghai, China). All aqueous solutions were prepared with bottled pure water (Wahaha, Hangzhou, China). High performance liquid chromatography grade acetonitrile and methanol were purchased from Fisher Scientific (Fair Lawn, NJ, USA). Other chemicals and solvents were all of analytical grade.

Shan J., Zhao X., Shen C., Ji J., Xu J., Wang S., Xie T, & Tong W. (2018). Liquid Chromatography Coupled with Linear Ion Trap Hybrid OrbitrapMass Spectrometry for Determination of Alkaloids in Sinomeniumacutum. Molecules : A Journal of Synthetic Chemistry and Natural Product Chemistry, 23(7), 1634.

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BTWT Herbal Components and Characterization

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The concentrated granules (with a concentrate to dried herb ratio of 10:0.9) of four component herbs of BTWT, R. pulsatilla, R. coptidis, C. phellodendri, and C. fraxini (lot numbers 8055671, 9010401, 1612619, and 7122571, respectively), were purchased from Guangdong Yifang Pharmaceutical Co., Ltd (Foshan, China). The structures of representative components from the four herbs are shown in Fig. 1. AB4, AA3, 23-HA, aesculin, aesculetin, palmatine, jatrorrhizine, coptisine, epiberberine, and phellodendrine were purchased from Yuanye Bio-Technology Co., Ltd at the highest available purity (98%) (Shanghai, China). Berberine hydrochloride was purchased from Shenzhen ChemStrong Scientific Co., Ltd (Shenzhen, China) at the highest available purity (95%). DSS was purchased from MP Biomedicals (MW; 36,000 − 50,000, Solon, OH, USA). The stool bleeding test kit was purchased from BaSO Bio-Technology Co., Ltd (Zhuhai, China). Lipopolysaccharide (LPS) was purchased from Sigma-Aldrich. Deionized water was purified using a Millipore water purification system (Millipore, Milford, MA, USA). All other reagents used were of analytical grade.Fig. 1

The label components of the four component herbs of BTWT

Deng L.R., Han Q., Zou M., Chen F.J., Huang C.Y., Zhong Y.M., Wu Q.Y., Tomlinson B, & Li Y.H. (2022). Identification of potential immunomodulators from Pulsatilla decoction that act on therapeutic targets for ulcerative colitis based on pharmacological activity, absorbed ingredients, and in-silico molecular docking. Chinese Medicine, 17, 132.

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Fluorometric Assay for Sialidase Activity

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NA from C. perfringens (Type V, 6 UN, lyophilized powder) and 2′-(4-methylumbelliferyl)-α-d-N-acetylneuraminic acid sodium salt hydrate (MUNANA) were purchased from Sigma–Aldrich (St. Louis, MO, USA). The BeaverBeads™ Mag NH₂ amine-terminated magnetic beads (2 μm, 10 mg/mL) were the products of Beaverbio Co., Ltd. (Suzhou, China). Pyridine, Tris(hydroxymethyl) methyl aminomethane (Tris), 4-morpholineethanesulfonic acid (MES), 50% glutaraldehyde, ammonium acetate, and dimethylsulfoxide (DMSO) were all obtained from Aladdin Chemistry (Shanghai, China). Standard compounds, including galantamine, jatrorrhizine, ga luteolin, quercetin, luteolin, and isorhamnetin were obtained from Shanghai Yuanye biotechnology Co., Ltd. (Shanghai, China). Acetonitrile and methanol (HPLC grade) were purchased from Merck (Germany). Deionized water purified by a Milli-Q water-purification system from Millipore (Bedford, MA, USA) was used for reagent and sample preparation and dilution. All buffer mentioned in this article were freshly prepared before experiments.

Luo S., Guo L., Sheng C., Zhao Y., Chen L., Li C., Jiang Z, & Tian H. (2020). Rapid identification and isolation of neuraminidase inhibitors from mockstrawberry (Duchesnea indica Andr.) based on ligand fishing combined with HR-ESI-Q-TOF-MS. Acta Pharmaceutica Sinica. B, 10(10), 1846-1855.

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Composition and Characterization of TAZF

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Carbamazepine (CAS No.: 298–46-4), berberine (CAS No.: 2086-83-1), coptisine (CAS No.: 6020-18-4), epiberberine (CAS No.: 6873-09-2), palmatine (CAS No.: 3486-67-7), jatrorrhizine (CAS No.: 3621-38-3), columbamine (CAS No.: 483–34-1), evodiamine (CAS No.: 518–17-2) and rutaecarpine (CAS No.: 20575–76-2), all > 98% purity, were purchased from Shanghai Yuanye Bio-Technology Company. Acetonitrile and methanol (LC grade) were purchased from Fisher Chemical, USA. Formaldehyde (AR) was purchased from Beijing Tong Guang Fine Chemicals Company. Isoflurane was provided by HARVEYBIO Company.
TAZF was provided by the State Key Laboratory of Natural and Biomimetic Drugs, School of Pharmaceutical Sciences, Peking University. The concentrations of the compounds in TAZF were measured using high-performance liquid chromatography (HPLC), with berberine accounting for the majority of TAZF at a ratio of 14.73% (g/g), followed by palmatine (3.14%), coptisine (2.52%), evodiamine (1.41%), epiberberine (1.30%), rutaecarpine (1.27%), columbamine (0.90%) and jatrorrhizine (0.47%).

Wang S., Zhang T., Liu X., Yang Z., Li L., Shan D., Gao Y., Li Y., Li Y., Zhang Y, & Wang Q. (2022). Toxicity and toxicokinetics of the ethanol extract of Zuojin formula. BMC Complementary Medicine and Therapies, 22, 220.

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Herbal Compound Evaluation for Inflammatory Bowel Disease

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XLP was purchased from the Hubei Xianglian Pharmaceutical Co., Ltd. (Z42020398, Hubei, China, the compositions were shown in Table 1). Standard solutions of berberine, jatrorrhizine, costunolide, coptisine, and palmatine were purchased from Yuanye Bio-Technology Co., Ltd (Shanghai, China, cat# B21449, B21451, B20891, B21438, B21433). DSS (36,000–50,000 MW) was purchased from MP Biomedicals (Santa Ana, CA, United States, cat# 160110). 5-aminosalicylic acid (5-ASA) was purchased from Sigma-Aldrich (St. Louis, MO, United States, cat#A79809). 3-methyladenine (3-MA) was purchased from Selleck (Huston, TX, United States, cat#S2767).

Wang B., Gong Z., Zhan J., Yang L., Zhou Q, & Yuan X. (2021). Xianglian Pill Suppresses Inflammation and Protects Intestinal Epithelial Barrier by Promoting Autophagy in DSS Induced Ulcerative Colitis Mice. Frontiers in Pharmacology, 11, 594847.

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