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α5hmc

Manufactured by Active Motif
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α5hmC is a laboratory equipment product that measures the levels of 5-hydroxymethylcytosine (5hmC) in DNA samples. It provides quantitative data on this epigenetic modification without further interpretation.

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Lab products found in correlation

β actin, by Abcam (1 mentions) α h3k27me3, by Merck Group (1 mentions) α tubulin, by Merck Group (1 mentions) α cleaved caspase 3, by Abcam (1 mentions) α β actin, by Merck Group (1 mentions) α flag, by Merck Group (1 mentions) Protease inhibitor cocktail, by Roche (1 mentions) 13c4 fumarate, by Cambridge Isotopes (1 mentions) 13c3 pyruvate, by Cambridge Isotopes (1 mentions) αcleaved caspase 8, by Cell Signaling Technology (1 mentions) α 6ma, by Synaptic Systems (1 mentions)

4 protocols using α5hmc

1

Comprehensive Protein Expression Analysis

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α-L2HGDH (Proteintech, #15707–1-AP, GeneTex, #GTX32695), α−5hmc (Active Motif, #39791), α-KDM6A (GeneTex, #GTX120873), α-MDH2 (Abcam, #ab96193), α-H3K27Me3 (Millipore, #07–449), α-H3 (CST, #9715), α-EZH2 (CST, #5246) and β-actin (Abcam, #ab20272) were used as per manufacturers’ instructions.
Shelar S.B., Shim E.H., Brinkley G.J., Kundu A., Carobbio F., Poston T., Tan J., Parekh V., Benson D., Crossman D.K., Buckhaults P.J., Rakheja D., Kirkman R., Sato Y., Ogawa S., Dutta S., Velu S.E., Emberley E., Pan A., Chen J., Huang T., Absher D., Becker A., Kunick C, & Sudarshan S. (2018). Biochemical and Epigenetic Insights into L-2-Hydroxyglutarate, a Potential Therapeutic Target in Renal Cancer. Clinical cancer research : an official journal of the American Association for Cancer Research, 24(24), 6433-6446.
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2

Metabolic Profiling and Epigenetic Analysis

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The antibodies used were αCS (SAB2701077), αACO2 (HPA001097), αOGDH (HPA020347), αSUCLG2 (HPA046705), αSDHA (HPA041981), αFH (HPA025770), αMDH2 (HPA019716), αTubulin (T8328), αKAT8 (SAB1306205), αβ-actin (A5441), and αFLAG (F3165) from Sigma; αIDH3A (ab228596), αSDHC (ab191362), αLamin B1 (ab133741), αH3K4me3 (ab8580), αH3K9me3 (ab8898), αH3K9ac (ab4441), αH3K27ac (ab4729), αpan-H3ac (ab47915), αH4K16ac (ab109463), αpan-H4ac (ab240201), αH3 (ab195277), αH4 (ab10158), αcleaved caspase-3 (ab32042), αFADD (ab108601), αKAT2A (ab217876), and αRUNX3 (ab224641) from Abcam; αcleaved caspase-8 (#9496) and αcleaved PARP (#5625) from Cell Signaling Technology; αPOLG (sc-390634) from Santa Cruz; α5hmC (#39069) from Active Motif; αZNF516 (A303-392A) from Bethyl; αMRPL39 (28165-1-AP) from Proteintech. Recombinant human KAT8 protein (SRP5220) was from Sigma and recombinant human KAT2A protein (ab198084) was from Abcam. Protease inhibitor cocktail was from Roche Applied Science. 13C3-pyruvate, 13C6-citrate, 13C4-fumarate and 13C4-malate were from Cambridge Isotope Laboratories.
Liu X., Si W., He L., Yang J., Peng Y., Ren J., Liu X., Jin T., Yu H., Zhang Z., Cheng X., Zhang W., Xia L., Huang Y., Wang Y., Liu S., Shan L., Zhang Y., Yang X., Li H., Liang J., Sun L, & Shang Y. (2021). The existence of a nonclassical TCA cycle in the nucleus that wires the metabolic-epigenetic circuitry. Signal Transduction and Targeted Therapy, 6, 375.
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3

Epigenetic Modification Detection Protocol

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The following antibodies were used: α6mA (Synaptic Systems, 202 003), α6mA (Megabase Research), α5mC (Active Motif, 39649), α5hmC (Active Motif 39769), α3mC (Active Motif, 61111 and 61179), and α1mA (Active Motif, custom). α6mA (Megabase Research) was only used in Figure S1A.
Greer E.L., Blanco M.A., Gu L., Sendinc E., Liu J., Aristizábal-Corrales D., Hsu C.H., Aravind L., He C, & Shi Y. (2015). DNA methylation on N6-adenine in C. elegans. Cell, 161(4), 868-878.
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4

Rabbit α-5-hmC Immunoprecipitation

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Rabbit α-5-hmC (39791; Active Motif), 10 μg per hMeDIP.
Wang X., Rosikiewicz W., Sedkov Y., Martinez T., Hansen B.S., Schreiner P., Christensen J., Xu B., Pruett-Miller S.M., Helin K, & Herz H.M. (2021). PROSER1 mediates TET2 O-GlcNAcylation to regulate DNA demethylation on UTX-dependent enhancers and CpG islands. Life Science Alliance, 5(1), e202101228.
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